文献类型： 外文期刊

作者： He, Yubing ¹ ; Mudgett, Michael ⁴ ; Zhao, Yunde ⁴ ;

作者机构： 1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

2.Nanjing Agr Univ, Collaborat Innovat Ctr Modern Crop Prod Cosponsor, Nanjing 210095, Peoples R China

3.Jiangsu Acad Agr Sci, Excellence & Innovat Ctr, Nanjing 210014, Peoples R China

4.Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA

期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.005； 五年影响因子：9.115 ）

ISSN： 0032-0889

年卷期： 2022 年 188 卷 4 期

页码：

收录情况： SCI

摘要： Transgene residuals in edited plants affect genetic analysis, pose off-target risks, and cause regulatory concerns. Several strategies have been developed to efficiently edit target genes without leaving any transgenes in plants. Some approaches directly address this issue by editing plant genomes with DNA-free reagents. On the other hand, DNA-based techniques require another step for ensuring plants are transgene-free. Fluorescent markers, pigments, and chemical treatments have all been employed as tools to distinguish transgenic plants from transgene-free plants quickly and easily. Moreover, suicide genes have been used to trigger self-elimination of transgenic plants, greatly improving the efficiency of isolating the desired transgene-free plants. Transgenes can also be excised from plant genomes using site-specific recombination, transposition or gene editing nucleases, providing a strategy for editing asexually produced plants. Finally, haploid induction coupled with gene editing may make it feasible to edit plants that are recalcitrant to transformation. Here, we evaluate the strengths and weaknesses of recently developed approaches for obtaining edited plants without transgene residuals. New technologies enable efficient isolation of target gene-edited plants without any transgene residuals.