The Function of Tryptophan Hydroxylase in Regulating Agonistic Behavior of the Chinese Mitten Crab (Eriocheir sinensis)
文献类型： 外文期刊
作者： Pang, Yangyang 1 ; Hou, Mengna 1 ; Zhang, Junyan 1 ; Zhang, Dongxin 1 ; Chen, Qing 1 ; Ge, Yongchun 3 ; Wang, Xinhai 4 ; Cheng, Yongxu 1 ; Yang, Xiaozhen 1 ;
作者机构： 1.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Engn Res Ctr Aquaculture, Key Lab Freshwater Aquat Genet Resources,Minist A, Shanghai 201306, Peoples R China
2.Chinese Acad Sci, South China Sea Inst Oceanol, State Key Lab Trop Oceanog, Guangzhou 510301, Peoples R China
3.Luoma Lake Crab Ind Res Inst, Suqian 223800, Peoples R China
4.Jiangsu Acad Agr Sci, Suqian Inst Agr Sci, Suqian 223800, Peoples R China
关键词： Eriocheir sinensis; agonistic behavior; l-tryptophan; tryptophan hydroxylase; serotonin
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2025 年 73 卷 19 期
页码：
收录情况： SCI
摘要： Agonistic behavior is common among crustaceans, but it presents risks such as energy loss, injury, and even death in aquaculture settings. Our previous study indicated that l-tryptophan (l-TRP) inhibits agonistic behavior in Eriocheir sinensis. However, supplementing l-TRP in diets increases costs and reduces nutritional quality. This study explores the role of tryptophan hydroxylase (TPH) in l-TRP metabolism and its regulation of agonistic behavior in E. sinensis. Our results show that l-TRP supplementation significantly increases the serotonin level and TPH expression. Subcellular localization confirmed the expression of the TPH in the cytoplasm of HEK293T cells. RNA interference of TPH significantly enhanced in the contact numbers of the crabs. While overexpression of the TPH significantly inhibited agonistic behavior in E. sinensis. Specifically, the fight duration was reduced from 6.67 +/- 2.03 min in the control group to 1.81 +/- 0.39 min, and the fight number decreased from 17 +/- 2.47 to 8.0 +/- 1.27. Moreover, the concentration of serotonin in E. sinensis was increased after TPH overexpression. Additionally, fluoxetine injection also significantly inhibited the agonistic behavior. In summary, our findings suggest that TPH regulates agonistic behavior in E. sinensis by mediating the conversion of l-TRP into 5-HT.
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