文献类型： 外文期刊

作者： Ma Qiao-li ¹ ; Kang Jun-mei ² ; Long Rui-cai ² ; Cui Yan-jun ² ; Zhang Tie-jun ² ; Xiong Jun-bo ² ; Yang Qing-chuan ² ; S ¹ ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

3.Ningxia Univ, Sch Chem & Chem Engn, Yinchuan 750021, Peoples R China

4.Hubei Acad Agr Sci, Inst Anim Sci, Wuhan 430064, Peoples R China

关键词： alfalfa;salinity stress;osmotic stress;seedling growth;proteomics

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2016 年 15 卷 10 期

页码：

收录情况： SCI

摘要： Alfalfa is widely grown and is one of the most important forage crops in the world, but its growth and biomass production are markedly reduced under salt and drought stress, particularly during the early seedling stage. To identify the mechanisms behind salt and drought responsiveness at the alfalfa seedling stage, the proteins expressed were analyzed under no-treatment, 200 mol L-1 NaCl and 180 g L-1 PEG treatment conditions during the seedling stage. Out of more than 800 protein spots detected on two-dimensional electrophoresis (2-DE) gels, 35 proteins showed statistically significant responses (P<0.05) to NaCl and PEG stress, which were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and 32 proteins were positively identified. The identified proteins were divided into seven functional categories: photosynthetic metabolism, protein biosynthesis, folding and assembly, carbohydrate metabolism-associated proteins, stress defense related protein, metabolism of nucleic acid, other function categories and unknown proteins. Our results suggested that these proteins may play roles in alfalfa adaptation to salt and drought stress. Further study of these proteins will provide insights into the molecular mechanisms of abiotic stress and the discovery of new candidate markers in alfalfa.