Mapping the Phosphorylation Regulatory Network of Single-Celled Fibre Elongation Mediated by GhBIN2 in Cotton
文献类型： 外文期刊
作者： Liu, Le 1 ; Yu, Mengli 1 ; Wang, Longfei 3 ; Qanmber, Ghulam 4 ; Fan, Liqiang 1 ; Li, Yujun 1 ; Liu, Zhao 4 ; Wang, Xuwen 5 ; Liu, Li 5 ; Li, Fuguang 1 ; Jin, Shuangxia 2 ; Yang, Zuoren 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biobreeding & Integrated Util, Anyang, Henan, Peoples R China
2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Hubei, Peoples R China
3.CAAS, Xinjiang Key Lab Crop Gene Editing & Germplasm Inn, Inst Western Agr, Changji, Xinjiang, Peoples R China
4.Zhengzhou Univ, Sch Agr Sci, State Key Lab Cotton Biobreeding & Integrated Util, Zhengzhou Res Base, Zhengzhou, Henan, Peoples R China
5.Xinjiang Acad Agr & Reclamat Sci, Cotton Res Inst, Northwest Inland Reg Key Lab Cotton Biol & Genet B, Shihezi, Xinjiang, Peoples R China
关键词： 4D-fastDIA quantitative phosphoproteomics; Brassinosteroids; cotton fibre; GhBIN2
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.5； 五年影响因子：12.4 ）
ISSN： 1467-7644
年卷期： 2025 年
页码：
收录情况： SCI
摘要： Protein phosphorylation plays a pivotal role in cellular signal transduction and plant development. The plant steroid hormone Brassinosteroids (BRs) signal transduction relies primarily on protein kinase-mediated phosphorylation cascades. However, the specific mechanisms of phosphorylation regulation in BR signalling remain to be fully elucidated. This study focuses on BIN2, an indispensable protein kinase in the BR signalling pathway, utilising single-celled cotton fibre to investigate the mechanisms by which phosphorylation regulates cell elongation. Firstly, we confirmed the inhibitory role of GhBIN2 in fibre elongation through its overexpression. Subsequently, we employed 4D-fastDIA quantitative phosphoproteomics and proteomics analysis to map the GhBIN2-mediated phosphorylation regulatory network. Through a comprehensive analysis of this network, we identified six credible substrates of GhBIN2. Further investigation revealed that GhBIN2 interacts with substrate GhIQD14 and increases its abundance through phosphorylation to negatively regulate fibre elongation. This study deepens the understanding of BR signalling in cotton fibre elongation and provides experimental evidence and new insights for comprehending the regulatory role of protein phosphorylation in plant cell elongation processes.
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