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Genome-wide analysis of WRKY gene family in high-CBD hemp (Cannabis sativa L.) and identification of the WRKY genes involved in abiotic stress responses and regulation cannabinoid accumulation

文献类型： 外文期刊

作者： Yu, Jiangshan 1 ; Cao, Xue 1 ; Mi, Yaolei 1 ; Sun, Wei 1 ; Meng, Xiangxiao 1 ; Chen, Weiqiang 1 ; Xie, Xuehua 1 ; Wang, Sifan 1 ; Li, Jun 1 ; Yang, Wei 1 ; Chen, Sha 1 ; Chen, Shilin 1 ; Xu, Wan 1 ; Wan, Huihua 1 ;

作者机构： 1.China Acad Chinese Med Sci, Inst Chinese Mat Med, State Key Lab Qual Ensurance & Sustainable Use Dao, Key Lab Beijing Identificat & Safety Evaluat Chine, Beijing 100070, Peoples R China

2.Chengdu Univ Tradit Chinese Med, Inst Herbgen, Chengdu 611137, Peoples R China

3.Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China

4.Zhejiang Acad Agr Sci, Zhejiang Inst Subtrop Crops, Wenzhou 325005, Peoples R China

关键词： WRKY; Hemp; Abiotic stress; Cannabinoids

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.9； 五年影响因子：6.0 ）

ISSN： 0926-6690

年卷期： 2024 年 210 卷

页码：

收录情况： SCI

摘要： Cannabinoids are vital metabolites produced by Cannabis sativa. The growing market demand for cannabinoids has made C. sativa a prominent research focus. The biosynthetic pathway of cannabinoids has been extensively investigated, but the information on the transcription factors (TFs) involved in cannabinoids biosynthesis remains limited. In this study, a total of 48 CsWRKYS were identified in high-cannabidiol (CBD) hemp using a combination of two-way blast, plantTFDB prediction, and CDD batching methods. These CsWRKYs exhibited uneven distribution across ten chromosomes, and encoded 162-728 amino acids. Phylogenetic analysis revealed that CsWRKY genes were categorized into three groups, with group II further divided into five subgroups. Members within each subfamily exhibited similar conserved motifs and exon-intron structures. Furthermore, gene expression analysis in various tissues, accompanied by cis-regulatory elements analysis, and alternative splicing analysis, provided insight into the potential roles of CsWRKYs in hemp growth and development. Notably, the expression of group III CsWRKY genes (CsWRKY1/2/20/26/28) was induced by salt, drought, and cold stress treatments. Metabolic analysis revealed an increase in the contents of cannabinoids under these treatments. Furthermore, CsWRKY20 and CsWRKY28 were proved to be positive regulatory factors of cannabinoids biosynthesis pathway. Altogether, the results of this study significantly contributes to understand the function of WRKY genes in response to abiotic stress, and their involvement in the transcriptional regulation of cannabinoid biosynthesis in high-CBD hemp.

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