Simple and rapid method for molecular identification of Konosirus punctatus and Clupanodon thrissa
文献类型： 外文期刊
作者： Li, Wei 1 ; Lei, Luo 1 ; Zhu, Junxian 1 ; Chen, Haigang 1 ; Chen, Chen 1 ; Wang, Yakun 1 ; Geng, Lulu 1 ; Chen, Fangcan 3 ; Zhu, Xinping 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr & Rural Affairs, Guangzhou 510380, Guangdong, Peoples R China
2.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 21408, Jiangsu, Peoples R China
3.Guangzhou Qianjiang Water Ecol Technol Co Ltd, Guangzhou 510380, Guangdong, Peoples R China
关键词： Konosirus punctatus; Clupanodon thrissa; mtDNA; Molecular marker; Variety identification
期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.991； 五年影响因子：0.897 ）
ISSN： 1877-7252
年卷期： 2022 年 14 卷 2 期
页码：
收录情况： SCI
摘要： Konosirus punctatus and Clupanodon thrissa are distributed in overlapping geographical areas. Their similar appearance makes it difficult to distinguish them by morphology. In recent years, overfishing has led to frequent stock collapses. The development of resource management and identification techniques is necessary for accurate statistical assessment of harvests and to protect the diversity of species and genetic resources. Considering the difficulty of distinguishing these two species, a molecular marker technique was developed to identify them. As a result, primer HB3 was developed to obtain amplification products for C. thrissa, which are not produced for K. punctatus. This study allows rapid distinction between these two species based on amplification products, providing a simple and rapid molecular method for checking products sold and illegally caught.
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