您好，欢迎访问海南省农业科学院 机构知识库！
海南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

IDENTIFICATION OF RICE ROOT KNOT NEMATODE IN HAINAN AND CLONING, LOCALIZATION AND SILENCING ANALYSIS OF FLP GENES

文献类型： 外文期刊

作者： Fu, Meiying 1 ; Luo, Jiguang 1 ; Zeng, Tao 2 ; Rui, Kai 1 ; Wang, Huifang 1 ; Ji, Xuncong 1 ; Chen, Miancai 1 ;

作者机构： 1.Hainan Acad Agr Sci, Inst Plant Protect, Key Lab Plant Dis & Pest Control Hainan Prov, Haikou 571100, Hainan, Peoples R China

2.Hainan Univ, Coll Trop Crops, Haikou 570228, Hainan, Peoples R China

关键词： Meloidogyne graminicola; Identification; Cloning; In situ hybridization; RNAi

期刊名称：FRESENIUS ENVIRONMENTAL BULLETIN （ 影响因子：0.489； 五年影响因子：0.479 ）

ISSN： 1018-4619

年卷期： 2021 年 30 卷 4 期

页码：

收录情况： SCI

摘要： Meloidogyne graminicola is one of the plant parasitic nematodes that affect rice yield most. In recent years, the occurrence area of rice root-knot nematode disease in rice growing areas in China has been increasing year by year. becoming a major root disease that restricts rice quality and yield. Destruction of neuropeptide signals of nematodes can disrupt their behavior and is one of the effective ways to control nematodes populations. In this study. M. graminicola was used as the object, and the simple collected from the field in Hainan island were identified. Four flp genes of M. graminicola were cloned and their functions were analyzed by in situ hybridization mapping and RNAi technology. The population 1MSY was identified as M. graminicola by morphological observation, molecular biological technology and homology analysis; four flp genes of If graminicola, including Mg-flp-1 (GenBank accession number MK886770), Mg-flp-13 (GenBank accession number MK886771), Mg-flp-14 and Mg-flp-18 fragments were cloned by RT-PCR, and their fragment sizes were 493 hp, 269 hp, 673 hp, 721 by respectively. The full length of Mg-flp-14 gene 1,047 bp (GenBank accession number MK855119) and the Mg-flp-18 gene's 3'end sequence 931 hp (GenBank accession number MK886769) were amplified by RACE technology. Mapping analysis of Mg-flp-1, Mg-flp-13, Mg-flp-14, and Mg-flp-18 genes of M. graminicola was carried out by in situ hybridization; RNAi technology was used to detect the silencing effect of Mg-flp-1, Mg-flp-13, Mg-flp-14 and Mg-flp-18 genes. Flp gene has the potential to control broad-spectrum nematodes to manage root-knot nematode disease in rice field planting system.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：海南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台