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Comparative Transcriptome Profiling of the Early Infection of Wheat Roots by Gaeumannomyces graminis var. tritici

文献类型： 外文期刊

作者： Yang, Lirong 1 ; Xie, Lihua 1 ; Xue, Baoguo 1 ; Goodwin, Paul H. 2 ; Quan, Xin 1 ; Zheng, Chuanlin 3 ; Liu, Taiguo 4 ; Le 1 ;

作者机构： 1.Henan Acad Agr Sci, Plant Protect Res Inst, Henan Key Lab Control Crop Dis & Insect Pests, IPM Key Lab Southern Part North China,Minist Agr, Zhengzhou, Peoples R China

2.Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada

3.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China

4.Chinese Acad Agr Sci, Inst Plant Protect, Bei

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2015 年 10 卷 4 期

页码：

收录情况： SCI

摘要： Take-all, which is caused by the fungal pathogen, Gaeumannomyces graminis var. tritici (Ggt), is an important soil-borne root rot disease of wheat occurring worldwide. However, the genetic basis of Ggt pathogenicity remains unclear. In this study, transcriptome sequencing for Ggt in axenic culture and Ggt-infected wheat roots was performed using Illumina paired-end sequencing. Approximately 2.62 and 7.76 Gb of clean reads were obtained, and 87% and 63% of the total reads were mapped to the Ggt genome for RNA extracted from Ggt in culture and infected roots, respectively. A total of 3,258 differentially expressed genes (DEGs) were identified with 2,107 (65%) being 2-fold up-regulated and 1,151 (35%) being 2-fold down-regulated between Ggt in culture and Ggt in infected wheat roots. Annotation of these DEGs revealed that many were associated with possible Ggt pathogenicity factors, such as genes for guanine nucleotide-binding protein alpha-2 subunit, cellulase, pectinase, xylanase, glucosidase, aspartic protease and gentisate 1, 2-dioxygenase. Twelve DEGs were analyzed for expression by qRT-PCR, and could be generally divided into those with high expression only early in infection, only late in infection and those that gradually increasing expression over time as root rot developed. This indicates that these possible pathogenicity factors may play roles during different stages of the interaction, such as signaling, plant cell wall degradation and responses to plant defense compounds. This is the first study to compare the transcriptomes of Ggt growing saprophytically in axenic cultures to it growing parasitically in infected wheat roots. As a result, new candidate pathogenicity factors have been identified, which can be further examined by gene knock-outs and other methods to assess their true role in the ability of Ggt to infect roots.

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