A Novel Transposon Tn7709 Harbors Multidrug Resistance Genes in a Pathogenic Aeromonas media Strain QST31
文献类型： 外文期刊
作者： Shang, Baodi 1 ; Li, Xiaoyi 1 ; Zhang, Xiaoping 1 ; Zhang, Meiyan 1 ; Kong, Jie 1 ; Wang, Jinle 1 ; Tan, Aiping 2 ; Zhao, Feng 1 ; Zhang, Defeng 2 ;
作者机构： 1.Guizhou Fisheries Res Inst, Guiyang 550025, Guizhou, Peoples R China
2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Minist Agr & Rural Affairs, Guangzhou 510380, Peoples R China
3.Guangdong Prov Key Lab Aquat Anim Immunol & Sustai, Guangzhou 510380, Peoples R China
关键词： Aeromonas media; antimicrobial resistance genes; transposon; integron; tadpoles
期刊名称：MICROORGANISMS （ 影响因子：4.5； 五年影响因子：4.8 ）
ISSN：
年卷期： 2024 年 12 卷 3 期
页码：
收录情况： SCI
摘要： Pathogenic Aeromonas spp. are the etiological agents of Motile Aeromonas Septicemia (MAS). This study aimed to identify the pathogen of diseased tadpoles (Quasipaa spinosa) and the antibiotic-resistance characteristics of this bacterium. A Gram-negative bacterium, named strain QST31, was isolated from the ascites of diseased tadpoles and was identified as Aeromonas media based on physiological and biochemical tests, as well as molecular identification. Artificial infection experiments showed that strain QST31 was highly virulent to tadpoles, with an LC50 of 2.56 x 10(7) CFU/mL. The antimicrobial susceptibility of strain QST31 was evaluated using the disk diffusion method, and the results indicated that strain QST31 was resistant to 28 antibacterial agents. In addition, the whole genome of strain QST31 was sequenced, and the presence of antimicrobial resistance genes, integron, and transposon was investigated. Genes involved in adherence, hemolysis, type II secretion system (T2SS), T6SS, iron uptake system, and quorum sensing were identified in the genome of strain QST31. More than 12 antimicrobial resistance genes were predicted in the genome of strain QST31. Interestingly, a novel Tn7709 transposon harboring sul1, aadA16, catB3, bla(OXA-21), aac(6 ')-IIa, and tet(A) genes was identified. In conclusion, this is the first report on the isolation and identification of pathogenic A. media with multidrug resistance genes from diseased tadpoles. The results revealed that preventing and controlling aquatic animal diseases caused by multidrug resistance A. media will be a huge challenge in the future.
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