文献类型： 外文期刊

作者： Wei, C. -B. ¹ ; Wang, J. -Q. ³ ; Chen, F. -Y. ² ; Niu, H. ³ ; Li, K. ¹ ;

作者机构： 1.Nanjing Agr Univ, Dept Anim Genet Breeding & Reprod, Coll Anim Sci & Technol, Nanjing, Jiangsu, Peoples R China

2.Henan Acad Agr Sci, Inst Anim Husb & Vet Med, Zhengzhou, Henan, Peoples R China

3.Res Ctr Cattle Engn Technol Henan, Zhengzhou, Henan, Peoples R China

4.Chinese Acad Agr Sci, Key Lab Domest Anim Genet Resources & Breeding, Minist Agr China, Inst Anim Sci, Beijing 100193, Peoples R China

关键词： AMPD1 gene;Cattle;Deletion mutation;Production traits

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 1 期

页码：

收录情况： SCI

摘要： The objectives of the present study were to detect an 18-bp deletion mutation in the bovine adenosine monophosphate deaminase 1 (AMPD1) gene and analyze its effect on growth traits in 2 Chinese cattle breeds using DNA sequencing and agarose electrophoresis. The five 19-bp polymerase chain reaction products of the AMPD1 gene exhibited 3 genotypes and 2 alleles: WW: homozygote genotype (wild-type); DD: homozygote genotype (mutant-type); WD: heterozygote genotype. Frequencies of the W allele varied from 66.15-70.35%. The associations between the 18-bp deletion mutation in the AMPD1 gene with production traits in 226 Jia-Xian red cattle was analyzed. The animals with genotype WW showed significantly higher heart girth and body weight than those with genotypes WD and DD at 24 months (P < 0.01). Our results indicate that the deletion mutation in the AMPD1 gene is associated with production traits, and may be used for marker-assisted selection in beef cattle breeding programs.