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CsiLAC4 modulates boron flow in Arabidopsis and Citrus via high-boron-dependent lignification of cell walls

文献类型： 外文期刊

作者： Huang, Jing-Hao 1 ; Zhang, Ling-Yuan 3 ; Lin, Xiong-Jie 1 ; Gao, Yuan 4 ; Zhang, Jiang 2 ; Huang, Wei-Lin 1 ; Zhao, Da 1 ;

作者机构： 1.Fujian Acad Agr Sci, Pomol Inst, Fuzhou 350013, Peoples R China

2.Fujian Agr & Forestry Univ, Coll Resources & Environm, Inst Plant Nutr Physiol & Mol Biol, Fuzhou 350002, Peoples R China

3.Fujian Univ Tradit Chinese Med, Fuzhou 350122, Peoples R China

4.Fujian Agr & Forestry Univ, Coll Hort, Fuzhou 350002, Peoples R China

5.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China

6.Univ Georgia, Dept Hort, Athens, GA 30602 USA

7.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou 350013, Peoples R China

8.Fujian Agr & Forestry Univ, Coll Resources & Environm, Fujian Prov Key Lab Soil Environm Hlth & Regulat, Fuzhou 350002, Peoples R China

关键词： boron toxicity; cell walls; Citrus; laccase-like protein; nutrient transport; nutrient uptake

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.152； 五年影响因子：10.476 ）

ISSN： 0028-646X

年卷期：

页码：

收录情况： SCI

摘要： The mechanisms underlying plant tolerance to boron (B) excess are far from fully understood. Here we characterized the role of the miR397-CsiLAC4/CsiLAC17 (from Citrus sinensis) module in regulation of B flow. Live-cell imaging techniques were used in localization studies. A tobacco transient expression system tested modulations of CsiLAC4 and CsiLAC17 by miR397. Transgenic Arabidopsis were generated to analyze the biological functions of CsiLAC4 and CsiLAC17. CsiLAC4's role in xylem lignification was determined by mRNA hybridization and cytochemistry. In situ B distribution was analyzed by laser ablation inductively coupled plasma mass spectrometry. CsiLAC4 and CsiLAC17 are predominantly localized in the apoplast of tobacco epidermal cells. Overexpression of CsiLAC4 in Arabidopsis improves the plants' tolerance to boric acid excess by triggering high-B-dependent lignification of the vascular system's cell wall and reducing free B content in roots and shoots. In Citrus, CsiLAC4 is expressed explicitly in the xylem parenchyma and is modulated by B-responsive miR397. Upregulation of CsiLAC4 in Citrus results in lignification of the xylem cell walls, restricting B flow from xylem vessels to the phloem. CsiLAC4 contributes to plant tolerance to boric acid excess via high-B-dependent lignification of cell walls, which set up a 'physical barrier' preventing B flow.

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