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Expression of lima bean terpene synthases in rice enhances recruitment of a beneficial enemy of a major rice pest

文献类型： 外文期刊

作者： Li, Fengqi 2 ; Li, Wei 3 ; Lin, Yong-Jun 3 ; Pickett, John A. 4 ; Birkett, Michael A. 4 ; Wu, Kongming 1 ; Wang, Guiro 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, 2 West Yuanmingyuan Rd, Beijing 100193, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

3.Huazhong Agr Univ, 1 Shizishan St, Wuhan 430070, Hubei, Peoples R China

4.Rothamsted Res, Dept Biol Chem & Crop Protect, Harpenden AL5 2JQ, Herts, England

关键词： (E)-4;8-dimethyl-1;3;7-nonatriene (DMNT);parasitic wasp;transgenic rice

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.228； 五年影响因子：7.791 ）

ISSN： 0140-7791

年卷期： 2018 年 41 卷 1 期

页码：

收录情况： SCI

摘要： Volatile terpenoids play a key role in plant defence against herbivory by attracting parasitic wasps. We identified seven terpene synthase genes from lima bean, Phaseolus lunatus L. following treatment with either the elicitor alamethicin or spider mites, Tetranychus cinnabarinus. Four of the genes (Pltps2, Pltps3, Pltps4 and Pltps5) were up-regulated with their derived proteins phylogenetically clustered in the TPS-g subfamily and PlTPS3 positioned at the base of this cluster. Recombinant PlTPS3 was able to convert geranyl diphosphate and farnesyl diphosphate to linalool and (E)-nerolidol, the latter being precursor of the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT). Recombinant PlTPS4 showed a different substrate specificity and produced linalool and (E)-nerolidol, as well as (E,E)-geranyllinalool from geranylgeranyl diphosphate. Transgenic rice expressing Pltps3 emitted significantly more (S)-linalool and DMNT than wild-type plants, whereas transgenic rice expressing Pltps4 produced (S)-linalool, DMNT and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT). In laboratory bioassays, female Cotesia chilonis, the natural enemy of the striped rice stemborer, Chilo suppressalis, were significantly attracted to the transgenic plants and their volatiles. We further confirmed this with synthetic blends mimicking natural rice volatile composition. Our study demonstrates that the transformation of rice to produce volatile terpenoids has the potential to enhance plant indirect defence through natural enemy recruitment. Homoterpenes are semiochemicals which modify insect behaviour and which are synthesized by terpene synthases. We identified, by Next generation sequencing (NGS), and functionally characterized, several terpene synthase genes from the lima bean, Phaseolus lunatus, following infestation with either spider mites or the elicitor alamethicin. Two up-regulated genes were transformed into an elite rice variety, enhancing homoterpene production and attraction of the natural enemy of rice pests, the parasitoid wasp Cotesia chilonis. This work demonstrated that the transformation of rice to produce volatile terpenoids has the potential to enhance plant defence through beneficial enemy recruitment.

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