A novel domain-duplicated SlitFAR3 gene involved in sex pheromone biosynthesis in Spodoptera litura
文献类型： 外文期刊
作者： Zhang, Biyun 1 ; Li, Fengqi 2 ; Qu, Cheng 2 ; Duan, Hongxia 3 ; Fu, Yuejun 1 ; Luo, Chen 2 ;
作者机构： 1.Shanxi Univ, Minist Educ, Inst Biotechnol, Key Lab Chem Biol & Mol Engn, Taiyuan, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing, Peoples R China
3.China Agr Univ, Coll Sci, Innovat Ctr Pesticide Res, Dept Appl Chem, Beijing, Peoples R China
4.Shanxi Univ, Minist Educ, Inst Biotechnol, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China
5.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing 100097, Peoples R China
关键词： domain duplication; fatty acyl reductase; molecular docking; SlitFAR3; Spodoptera litura; yeast expression
期刊名称：INSECT SCIENCE （ 影响因子：4.0； 五年影响因子：3.6 ）
ISSN： 1672-9609
年卷期： 2023 年 30 卷 3 期
页码：
收录情况： SCI
摘要： Fatty acyl reductases (FARs) are key enzymes that participate in sex pheromone biosynthesis by reducing fatty acids to fatty alcohols. Lepidoptera typically harbor numerous FAR gene family members. Although FAR genes are involved in the biosynthesis of sex pheromones in moths, the key FAR gene of Spodoptera litura remains unclear. In this work, we predicted 30 FAR genes from the S. litura genome and identified a domain duplication within gene SlitFAR3, which exhibited high and preferential expression in the sexually mature female pheromone glands (PGs) and a rhythmic expression pattern during the scotophase of sex pheromone production. The molecular docking of SlitFAR3, as predicted using a 3D model, revealed a co-factor NADPH binding cavity and 2 substrate binding cavities. Functional expression in yeast cells combined with comprehensive gas chromatography indicated that the SlitFAR3 gene could produce fatty alcohol products. This study is the first to focus on the special phenomenon of FAR domain duplication, which will advance our understanding of biosynthesis-related genes from the perspective of evolutionary biology.
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