您好，欢迎访问宁夏农林科学院 机构知识库！
宁夏农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Engineered Dwarf Male-Sterile Rice: A Promising Genetic Tool for Facilitating Recurrent Selection in Rice

文献类型： 外文期刊

作者： Ansari, Afsana 1 ; Wang, Chunlian 1 ; Wang, Jian 1 ; Wang, Fujun 1 ; Liu, Piqing 3 ; Gao, Ying 1 ; Tang, Yongchao 1 ; Zha 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing, Peoples R China

2.Ningxia Acad Agr & Forestry Sci, Crop Inst, Yinchuan, Peoples R China

3.Guangxi Univ, Coll Agr, Nanning, Peoples R China

4.Bangladesh Rice Res Inst, Gazipur, Bangladesh

关键词： rice breeding;dwarf;male-sterile;emasculation;recurrent selection;RNAi;multiple gene manipulation

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Rice is a crop feeding half of the world's population. With the continuous raise of yield potential via genetic improvement, rice breeding has entered an era where multiple genes conferring complex traits must be efficiently manipulated to increase rice yield further. Recurrent selection is a sound strategy for manipulating multiple genes and it has been successfully performed in allogamous crops. However, the difficulties in emasculation and hand pollination had obstructed efficient use of recurrent selection in autogamous rice. Here, we report development of the dwarf male-sterile rice that can facilitate recurrent selection in rice breeding. We adopted RNAi technology to synergistically regulate rice plant height and male fertility to create the dwarf male-sterile rice. The RNAi construct pTCK-EGGE, targeting the OsGA20ox2 and OsEAT1 genes, was constructed and used to transform rice via Agrobacterium-mediated transformation. The transgenic T0 plants showing largely reduced plant height and complete male-sterile phenotypes were designated as the dwarf male-sterile plants. Progenies of the dwarf male-sterile plants were obtained by pollinating them with pollens from the wild-type. In the T1 and T2 populations, half of the plants were still dwarf male-sterile; the other half displayed normal plant height and male fertility which were designated as tall and male-fertile plants. The tall and male-fertile plants are transgene-free and can be self-pollinated to generate new varieties. Since emasculation and hand pollination for dwarf male-sterile rice plants is no longer needed, the dwarf male-sterile rice can be used to perform recurrent selection in rice. A dwarf male-sterile rice-based recurrent selection model has been proposed.

  • 相关文献

[1]OsGA20ox2不同长度RNAi片段对水稻株高等农艺性状的遗传效应. 王坚,赵开军,乔枫,杨生龙. 2012

[2]OsGA200x2不同长度RNAi片段对水稻株高等农艺性状的遗传效应. 王坚,赵开军,乔枫,杨生龙. 2012

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：宁夏农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台