您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterizing heat shock protein 90 gene of Apolygus lucorum (Meyer-Dur) and its expression in response to different temperature and pesticide stresses

文献类型： 外文期刊

作者： Sun, Yang 2 ; Sheng, Yang 1 ; Bai, Lixin 1 ; Zhang, Yongjun 2 ; Xiao, Yingfang 3 ; Xiao, Liubin 1 ; Tan, Yongan 1 ; Shen, 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Univ Florida, Midflorida Res & Educ Ctr, Apopka, FL 32703 USA

关键词： A. lucorum;HSP90;Pesticide;Extreme temperature;Temperature stress;Expression files

期刊名称：CELL STRESS & CHAPERONES （ 影响因子：3.667； 五年影响因子：3.717 ）

ISSN： 1355-8145

年卷期： 2014 年 19 卷 5 期

页码：

收录情况： SCI

摘要： In this study, we cloned a full-length cDNA of heat shock protein (HSP) gene of Apolygus lucorum (Meyer-Dur) [AlHSP90, KC109781] and investigated its expression in response to temperature and pesticide stresses. The open reading frame (ORF) of AlHSP90 is 2,169 bp in length, encoding a 722 amino acid polypeptide with a predicted molecular weight of 82.99 kDa. Transcriptional and translational expression profiles of AlHSP90 under extreme temperature or pesticide stresses were examined by fluorescent real-time quantitative PCR and Western blot. Results showed that the expression profiles of AlHSP90 protein were in high agreement with those of AlHSP90 RNA and indicated that AlHSP90 was not only an important gene for A. lucorum adults in response to extremely high temperature, but also involved in the resistance or tolerance to cyhalothrin, imidacloprid, chlorpyrifos, and emamectin benzoate, especially for female adults to emamectin benzoate and for male adults to cyhalothrin. Transcriptional results of AlHSP90 also confirmed that AlHSP90 was an important gene involved in the resistance or tolerance to both temperature and pesticide stresses. In addition, our study also revealed that similar to 24 A degrees C may be the suitable temperature range for A. lucorum survival, which is also confirmed by the results of the expression of AlHSP90, the nymph mortality, and the intrinsic rate of increase (r (m)) when A. lucorum is reared at six different temperatures. Therefore, these studies are significant in elucidating the AlHSP90 in response to temperature and pesticide stresses and would provide guidance for A. lucorum management with different pesticides or temperatures in fields.

  • 相关文献

[1]Identification of heat shock cognate protein 70 gene (Alhsc70) of Apolygus lucorum and its expression in response to different temperature and pesticide stresses. Zhao, Jing,Sheng, Yang,Bai, Li-Xin,Tan, Yongan,Xiao, Liu-Bin,Xu, Guang-Chun,Sun, Yang,Zhang, Yong-Jun,Xiao, Ying-Fang. 2016

[2]Molecular characterisation of the vitellogenin gene (AlVg) and its expression after Apolygus lucorum had fed on different hosts. Xiao, Liubin,Xu, Guangchun,Zhao, Jing,Tan, Yongan,Bai, Lixin,Sun, Yang,Cao, Guangchun,Zhang, Yongjun,Xiao, Yingfang.

[3]Graphite-like g-C3N4-F127-Au nanosheets used for sensitive monitoring of heat shock protein 90. Sun, Chong,Zhang, Muhan,Wang, Daoying,Sun, Zhilan,Geng, Zhiming,Xu, Weimin,Liu, Fang,Fei, Qiqi. 2018

[4]Interaction of heat shock protein 90 B1 (Hsp90B1) with liposome reveals its potential role in protection the integrity of lipid membranes. Li, Pengpeng,Zhang, Muhan,Zou, Ye,Sun, Zhilan,Sun, Chong,Geng, Zhiming,Xu, Weimin,Wang, Daoying. 2018

[5]Effect of heat shock protein 90 against ROS-induced phospholipid oxidation. Zhang, Muhan,Wang, Daoying,Geng, Zhiming,Li, Pengpeng,Sun, Zhilan,Xu, Weimin,Xu, Weimin. 2018

[6]Interaction of Hsp90 with phospholipid model membranes. Zhang, Muhan,Wang, Daoying,Li, Pengpeng,Sun, Chong,Xu, Rong,Geng, Zhiming,Xu, Weimin,Dai, Zhaoqi,Xu, Weimin. 2018

[7]The level of heat shock protein 90 in pig Longissimus dorsi muscle and its relationship with meat pH and quality. Zhang, Muhan,Wang, Daoying,Geng, Zhiming,Bian, Huan,Liu, Fang,Zhu, Yongzhi,Xu, Weimin.

[8]Recent progress of highly efficient in vivo biological screening for novel agrochemicals in China. Qian, Xuhong,Li, Baoju,Yuan, Huizhu,Fang, Jichao,Tao, Liming,Fan, Zhijin.

[9]Isolation and Identification of the DNA Aptamer Target to Acetamiprid. He, Jiang,Liu, Yuan,Liu, Xianjin,He, Jiang,Fan, Mingtao.

[10]Reduced plant uptake of pesticides with biochar additions to soil. Yu, Xiang-Yang,Ying, Guang-Guo,Kookana, Rai S.,Yu, Xiang-Yang,Ying, Guang-Guo.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台