您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Comparative Transcriptome Analysis of Two Races of Heterodera glycines at Different Developmental Stages

文献类型： 外文期刊

作者： Wang, Gaofeng 2 ; Peng, Deliang 1 ; Gao, Bingli 3 ; Huang, Wenkun 1 ; Kong, Lingan 1 ; Long, Haibo 1 ; Peng, Huan 1 ; Jia 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, Key Lab Biol Insect Pests & Plant Dis, Beijing 100193, Peoples R China

2.China Agr Univ, Dept Plant Pathol, Beijing 100094, Peoples R China

3.Chinese Acad Sci, Shanghai Inst Biol Sci, Huzhou Modem Agr Biotechnol Innovat Ctr, Huzhou, Zhejiang, Peoples R China

4.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Pests Comprehens Gov

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 3 期

页码：

收录情况： SCI

摘要： The soybean cyst nematode, Heterodera glycines, is an important pest of soybeans. Although resistance is available against this nematode, selection for virulent races can occur, allowing the nematode to overcome the resistance of cultivars. There are abundant field populations, however, little is known about their genetic diversity. In order to elucidate the differences between races, we investigated the transcriptional diversity within race 3 and race 4 inbred lines during their compatible interactions with the soybean host Zhonghuang 13. Six different race-enriched cDNA libraries were constructed with limited nematode samples collected from the three sedentary stages, parasitic J2, J3 and J4 female, respectively. Among 689 putative race-enriched genes isolated from the six libraries with functional annotations, 92 were validated by quantitative RT-PCR (qRT-PCR), including eight putative effector encoding genes. Further race-enriched genes were validated within race 3 and race 4 during development in soybean roots. Gene Ontology (GO) analysis of all the race-enriched genes at J3 and J4 female stages showed that most of them functioned in metabolic processes. Relative transcript level analysis of 13 selected race-enriched genes at four developmental stages showed that the differences in their expression abundance took place at either one or more developmental stages. This is the first investigation into the transcript diversity of H. glycines races throughout their sedentary stages, increasing the understanding of the genetic diversity of H. glycines.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台