Comparative Transcriptome Analysis of Two Races of Heterodera glycines at Different Developmental Stages
文献类型: 外文期刊
作者: Wang, Gaofeng 2 ; Peng, Deliang 1 ; Gao, Bingli 3 ; Huang, Wenkun 1 ; Kong, Lingan 1 ; Long, Haibo 1 ; Peng, Huan 1 ; Jia 1 ;
作者机构: 1.Chinese Acad Agr Sci, Inst Plant Protect, Key Lab Biol Insect Pests & Plant Dis, Beijing 100193, Peoples R China
2.China Agr Univ, Dept Plant Pathol, Beijing 100094, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Huzhou Modem Agr Biotechnol Innovat Ctr, Huzhou, Zhejiang, Peoples R China
4.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Pests Comprehens Gov
期刊名称:PLOS ONE ( 影响因子:3.24; 五年影响因子:3.788 )
ISSN: 1932-6203
年卷期: 2014 年 9 卷 3 期
页码:
收录情况: SCI
摘要: The soybean cyst nematode, Heterodera glycines, is an important pest of soybeans. Although resistance is available against this nematode, selection for virulent races can occur, allowing the nematode to overcome the resistance of cultivars. There are abundant field populations, however, little is known about their genetic diversity. In order to elucidate the differences between races, we investigated the transcriptional diversity within race 3 and race 4 inbred lines during their compatible interactions with the soybean host Zhonghuang 13. Six different race-enriched cDNA libraries were constructed with limited nematode samples collected from the three sedentary stages, parasitic J2, J3 and J4 female, respectively. Among 689 putative race-enriched genes isolated from the six libraries with functional annotations, 92 were validated by quantitative RT-PCR (qRT-PCR), including eight putative effector encoding genes. Further race-enriched genes were validated within race 3 and race 4 during development in soybean roots. Gene Ontology (GO) analysis of all the race-enriched genes at J3 and J4 female stages showed that most of them functioned in metabolic processes. Relative transcript level analysis of 13 selected race-enriched genes at four developmental stages showed that the differences in their expression abundance took place at either one or more developmental stages. This is the first investigation into the transcript diversity of H. glycines races throughout their sedentary stages, increasing the understanding of the genetic diversity of H. glycines.
- 相关文献
作者其他论文 更多>>
-
Detection and molecular characterization of the seville root-knot nematode Meloidogyne hispanica parasiting pepper in China
作者:Wang, Li;Wang, Li;Chen, Yuan;Wang, Shuaili;Feng, Tuizi;Sun, Yanfang;Long, Haibo
关键词:Root-knot nematode; Meloidogyne hispanica; Pepper; Characterization
-
Identification and molecular characterization of two novel viruses from Allamanda cathartica in China
作者:Che, Haiyan;Ma, Yuxin;Long, Haibo;Che, Haiyan;Long, Haibo;Wu, Yangxin
关键词:
-
Morphological and Molecular Characterization of a New Root-Knot Nematode, Meloidogyne limonae n. sp. (Nematoda: Meloidogynidae), Parasitizing Lemon in China
作者:Sun, Yangfang;Long, Haibo;Jiang, Ru;Peng, Deliang;Peng, Huan;Zhang, Yan
关键词:Citrus limon; histopathology; nematode taxonomy; root-knot nematode
-
As a Transitional Host, Weed Solanum nigrum L. Increases the Population Base of Root-Knot Nematode Meloidogyne enterolobii for the Next Season
作者:Pei, Yueling;Sun, Yanfang;Chen, Yuan;Feng, Tuizi;Che, Haiyan;Long, Haibo;Long, Haibo
关键词:Meloidogyne enterolobii; transitional host; Solanum nigrum L.; parasitic characteristics; population base
-
Brown planthopper infestation on rice reduces plant susceptibility to Meloidogyne graminicola by reducing root sugar allocation
作者:Xiao, Liying;Xiao, Xueqiong;Guo, Xiaoli;Yang, Lijie;Wang, Gaofeng;Xiao, Liying;Xiao, Xueqiong;Xu, Lihe;Guo, Xiaoli;Yang, Lijie;Ma, Kangsheng;Wang, Gaofeng;Xiao, Yannong;Gheysen, Godelieve;Yang, Mingwei;Liu, Wen;He, Yueping;Peng, Deliang;Peng, Huan;Long, Haibo
关键词:Nilaparvata lugens; oviposition; phloem-feeding; rice root-knot nematode; sugar allocation; tripartite interaction
-
Occurrence of Root-Knot Nematode (Meloidogyne spp.) on Peppers in Hainan, China, and M. enterolobii and M. incognita Resistance of Common Cultivars
作者:Long, Haibo;Sun, Yanfang;Chen, Yuan;Pei, Yueling;Feng, Tuizi;Che, Haiyan;Long, Haibo
关键词:Meloidogyne enterolobii; occurrence; pepper; resistance; root-knot nematodes
-
Association of Globodera rostochiensis (Nematoda) with Stunted and Chlorotic Potato Plants in Yunnan and Sichuan Provinces in China
作者:Jiang, Ru;Li, Yunqing;Huang, Liqiang;Liu, Shiming;Huang, Wenkun;Kong, Lin'an;Peng, Huan;Peng, Deliang;Liu, Hui;Feng, Xiaodong;Long, Haibo;Hu, Xianqi;Ge, Jianjun;Bian, Yong;Li, Xingyue;Shao, Baolin;Xu, Chong
关键词:cyst nematode; field crops; mitochondrial sequences; morphological characteristics; nematodes; pathogen detection; potato; rDNA sequences