文献类型： 外文期刊

作者： Yang, Tao ¹ ; Jiang, Junye ¹ ; Burlyaeva, Marina ² ; Hu, Jinguo ³ ; Coyne, Clarice J. ³ ; Kumar, Shiv ⁴ ; Redden, Robe ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

2.NI Vavilov Res Inst Plant Ind, Dept Leguminous Crops Genet Resources, St Petersburg 190000, Russia

3.Washington State Univ, USDA ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA

4.Int Ctr Agr Res Dry Areas, A

关键词： Lathyrus sativus L;Microsatellite;454 FLX Titanium pyrosequencing;Marker development

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2014 年 14 卷

页码：

收录情况： SCI

摘要： Background: Grasspea (Lathyrus sativus L., 2n = 14), a member of the family Leguminosae, holds great agronomic potential as grain and forage legume crop in the arid areas for its superb resilience to abiotic stresses such as drought, flood and salinity. The crop could not make much progress through conventional breeding in the past, and there are hardly any detailed molecular biology studies due to paucity of reliable molecular markers representative of the entire genome. Results: Using the 454 FLX Titanium pyrosequencing technique, 651,827 simple sequence repeat (SSR) loci were identified and 50,144 nonredundant primer pairs were successfully designed, of which 288 were randomly selected for validation among 23 L. sativus and one L. cicera accessions of diverse provenance. 74 were polymorphic, 70 monomorphic, and 144 with no PCR product. The number of observed alleles ranged from two to five, the observed heterozygosity from 0 to 0.9545, and Shannon's information index ranged from 0.1013 to 1.0980, respectively. The dendrogram constructed by using unweighted pair group method with arithmetic mean (UPGMA) based on Nei's genetic distance, showed obvious distinctions and understandable relationships among the 24 accessions. Conclusions: The large number of SSR primer pairs developed in this study would make a significant contribution to genomics enabled improvement of grasspea.