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Development of IMBs-qPCR detection method for Yersinia enterocolitica based on the foxA gene

文献类型： 外文期刊

作者： Shi, Jingxuan 1 ; Chi, Heng 1 ; Cao, Aiping 1 ; Song, Yinna 1 ; Zhu, Min 1 ; Zhang, Lilin 1 ; Xu, Fuzhou 2 ; Huang, Jinha 1 ;

作者机构： 1.Tianjin Univ, Sch Life Sci, China 92,Weijin Rd, Tianjin 300072, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing 100097, Peoples R China

关键词： Yersinia enterocolitica; Outer membrane protein; Immunomagnetic beads; Quantitative real-time PCR

期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：2.552； 五年影响因子：2.475 ）

ISSN： 0302-8933

年卷期： 2021 年 203 卷 7 期

页码：

收录情况： SCI

摘要： Yersinia enterocolitica is an important zoonotic pathogen, which seriously endangers food-safety risk. In this study, the recombinant outer membrane protein OmpF and its antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Y. enterocolitica in food samples, combining the quantitative PCR detection with primers of virulence factor gene foxA for Yersinia enterocolitica contamination. The results showed that the capture efficiency of approximately 80% using anti-OmpF antibody-immunomagnetic beads and linearly dependent capture under 10(1)-10(5) CFU/mL Y. enterocolitica compared with less than 10% capture of other bacteria. The detection limit of 64 CFU/mL was obtained based on foxA gene PCR detection combined with capture of the anti-OmpF antibody-immunomagnetic beads to detect Yersinia enterocolitica in artificially contaminated milk and pork samples. Compared to the culture method, the developed IMBs-qPCR method has higher consistency, was less time consuming, which taken together provides an effective alternative method for rapid detection of Y. enterocolitica in food.

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