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Phosphate Transporter OsPT4, Ubiquitinated by E3 Ligase OsAIRP2, Plays a Crucial Role in Phosphorus and Nitrogen Translocation and Consumption in Germinating Seed

文献类型： 外文期刊

作者： Sun, Yafei 1 ; Zhang, Fang 4 ; Wei, Jia 6 ; Song, Ke 1 ; Sun, Lijuan 1 ; Yang, Yang 1 ; Qin, Qin 1 ; Yang, Shiyan 1 ; Li, Zhouwen 1 ; Xu, Guohua 2 ; Sun, Shubin 2 ; Xue, Yong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Ecoenvironm & Plant Protect, Shanghai 201403, Peoples R China

2.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Key Lab Plant Nutr & Fertilizat Low Middle Reaches, Minist Agr, Nanjing 210095, Peoples R China

3.Shanghai Acad Agr Sci, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China

4.Jiangxi Agr Univ, Key Lab Crop Physiol Ecol & Genet Breeding, Jiangxi Key Lab Crop Physiol Ecol & Genet Breeding, Minist Educ, Nanchang 330045, Peoples R China

5.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

6.Jilin Acad Agr Sci, Jilin Prov Key Lab Agr Biotechnol, Changchun 130033, Peoples R China

关键词： Seed germination; OsPT4; Phosphate; Amino acid; Nitrogen

期刊名称：RICE （ 影响因子：5.5； 五年影响因子：5.8 ）

ISSN： 1939-8425

年卷期： 2023 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Phosphorus (P) and nitrogen (N) are essential macronutrients necessary for plant growth and development. OsPT4 is a high-affinity phosphate (Pi) transporter that has a positive impact on nutrient uptake and seed development. In this study, the expression patterns of different Pi transporter genes in germinating seeds were determined, and the relative expression of OsPT4 was induced in Pi-deficient seeds and gradually increased with the passage of germination time. The analysis of P, N, Pi, and amino acid concentrations in germinating seeds of OsPT4 mutants showed that the OsPT4 mutation caused P and N retention and a continuous reduction in multiple amino acid concentrations in germinating seeds. Transcriptome analysis and qRT-PCR results also indicated that the OsPT4 mutation inhibits the expression of genes related to P and N transportation and amino acid synthesis in germinating seeds. In addition, the paraffin section and TUNEL assay of OsPT4 mutant germinating seeds suggests that OsPT4 mutation causes programmed cell death (PCD) delayed in the aleurone layer and inhibition of leaf outgrowth. Moreover, we also found that OsPT4 was ubiquitinated by OsAIRP2, which is a C3HC4-type RING E3 Ub ligase. Our studies illustrate that OsPT4 plays a crucial role in P and N collaborative translocation and consumption in germinating seeds. It also provides a theoretical basis for the molecules and physiological mechanisms of P and N cross-talk under suppressed Pi uptake conditions.

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