文献类型： 外文期刊

作者： Yang, Xiaojie ¹ ; Li, Fuguang ¹ ; Zhang, Xueyan ¹ ; Liu, Kun ¹ ; Wang, Qianhua ¹ ; Zhang, Chaojun ¹ ; Liu, Chuanliang ¹ ; Zhu, Wei ¹ ; Shan, Guofang ¹ ; Chin, Chee-Kok ¹ ; Fang, Weiping ¹ ;

作者机构： 1.State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China;Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, P.R. China

2.State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China

3.Agronomy College of Henan Agricultural University, Zhengzhou, P.R. China

4.Department of Plant Science, Rutgers University, New Jersey, USA

5.Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, P.R. China

关键词： cotton (Gossypium hirsutum L.);deletion of border;genetic transformation;transgene copy;vector integration

期刊名称：CZECH JOURNAL OF GENETICS AND PLANT BREEDING （ 影响因子：0.865； 五年影响因子：1.017 ）

ISSN： 1212-1975

年卷期： 2013 年 49 卷 2 期

页码：

收录情况： SCI

摘要： Copy numbers were evaluated by real-time quantitative PCR, and 149 junctions of T-DNA were isolated by thermal asymmetric interlaced PCR from 92 independent transgenic cotton lines transformed by Agrobacterium tumefaciens strain LBA4404. Real-time quantitative PCR results showed that 46% had integration of one or two T-DNA copies, 54% had three or more copies. Among 63 amplified products at LB junctions, 51% showed cotransformation of the vector backbone, 30% retained a portion of LB ranging from 3 to 23 bp, and 19% showed deletions ranging from 1 to 148 bp from the LB inner end. In contrast, all of the cleavage sites were located in the inner region of RB. The distribution of T-DNA insertions in upland cotton genome included coding sequences, transposons, plastid-derived sequences and microsatellites.