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OPTIMIZATION OF FLASK CULTURE MEDIUM AND CONDITIONS FOR HYALURONIC ACID PRODUCTION BY A STREPTOCOCCUS EQUISIMILIS MUTANT NC2168

文献类型： 外文期刊

作者： Chen, Yong-Hao 1 ; Li, Jun 3 ; Liu, Li 2 ; Liu, Hong-Zhi 2 ; Wang, Qiang 2 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100093, Peoples R China

2.Chinese Acad Agr Sci, Inst Agrofood Sci & Technol, Beijing 100193, Peoples R China

3.Beijing Inst Landscape Architecture, Beijing 100102, Peoples R China

关键词： Streptococcus equisimilis mutant;hyaluronic acid;optimization;culture medium;culture conditions

期刊名称：BRAZILIAN JOURNAL OF MICROBIOLOGY （ 影响因子：2.476； 五年影响因子：3.391 ）

ISSN： 1517-8382

年卷期： 2012 年 43 卷 4 期

页码：

收录情况： SCI

摘要： A mutant designated NC2168, which was selected from wild-type Streptococcus equisimilis CVCC55116 by ultraviolet ray combined with C-60(o)-gamma ray treatment and does not produce streptolysin, was employed to produce hyaluronic acid (HA). In order to increase the output of HA in a flask, the culture medium and conditions for NC2168 were optimized in this study. The influence of culture medium ingredients including carbon sources, nitrogen sources and metal ions on HA production was evaluated using factional factorial design. The mathematical model, which represented the effect of each medium component and their interaction on the yield of HA, was established by the quadratic rotary combination design and response surface method. The model estimated that, a maximal yield of HA could be obtained when the concentrations of yeast extract, peptone, glucose, and MgSO4 were set at 3 g/100 mL, 2 g/100 mL, 0.5 g/100 mL and 0.15 g/100 mL, respectively. Compared with the values obtained by other runs in the experimental design, the optimized medium resulted in a remarkable increase in the output of HA and the maximum of the predicted HA production was 174.76 mg/L. The model developed was accurate and reliable for predicting the production of HA by NC2168. Cultivation conditions were optimized by an orthogonal experimental design and the optimal conditions were as follows: temperature 33 degrees C, pH 7.8, agitation speed 200 rpm, medium volume 20 mL.

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