Soybean Oil Regulates the Fatty Acid Synthesis II System of Bacillus amyloliquefaciens LFB112 by Activating Acetyl-CoA Levels
文献类型： 外文期刊
作者： Cheng, Qiang 1 ; Li, Zhongxuan 1 ; Zhang, Jing 1 ; Guo, Henan 1 ; Ahmat, Marhaba 1 ; Cheng, Junhao 1 ; Abbas, Zaheer 1 ; Hua, Zhengchang 1 ; Wang, Junyong 1 ; Tong, Yucui 1 ; Yang, Tiantian 1 ; Si, Dayong 1 ; Zhang, Rijun 1 ;
作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Lab Feed Biotechnol, Beijing 100193, Peoples R China
2.Sichuan Univ Sci & Engn, Coll Bioengn, Zigong 643000, Peoples R China
3.Xinjiang Acad Agr Sci, Inst Appl Microbiol, Xinjiang Lab Special Environm Microbiol, Urumqi 830091, Peoples R China
关键词： Bacillus LFB112; fatty acid metabolism; type II fatty acid synthesis pathway; unsaturated fatty acids
期刊名称：MICROORGANISMS （ 影响因子：4.5； 五年影响因子：4.8 ）
ISSN：
年卷期： 2023 年 11 卷 5 期
页码：
收录情况： SCI
摘要： [Background] Bacillus LFB112 is a strain of Bacillus amyloliquefaciens screened in our laboratory. Previous studies found that it has a strong ability for fatty acid metabolism and can improve the lipid metabolism of broilers when used as feed additives. [Methods] This study aimed to confirm the fatty acid metabolism of Bacillus LFB112. Sterilized soybean oil (SSO) was added to the Beef Peptone Yeast (BPY) medium, and its effect on fatty acid content in the supernatant and bacteria, as well as expression levels of genes related to fatty acid metabolism, were studied. The control group was the original culture medium without oil. [Results] Acetic acid produced by the SSO group of Bacillus LFB112 decreased, but the content of unsaturated fatty acids increased. The 1.6% SSO group significantly increased the contents of pyruvate and acetyl-CoA in the pellets. Furthermore, the mRNA levels of enzymes involved in the type II fatty acid synthesis pathway of FabD, FabH, FabG, FabZ, FabI, and FabF were up-regulated. [Conclusions] Soybean oil increased the content of acetyl-CoA in Bacillus LFB112, activated its type II fatty acid synthesis pathway, and improved the fatty acid metabolism level of Bacillus LFB112. These intriguing results pave the way for further investigations into the intricate interplay between Bacillus LFB112 and fatty acid metabolism, with potential applications in animal nutrition and feed additive development.
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