文献类型： 外文期刊

作者： Sun, Jia-Wen ¹ ; Liu, Li-Yuan ¹ ; Cui, Jia-Ning ¹ ; Zhai, Jia-Yue ¹ ; Zhang, Hai-Ling ² ; Wang, Quan-Wei ¹ ;

作者机构： 1.Harbin Normal Univ, Prov Key Lab Mol Genet & Genet Breeding, Harbin 150000, Peoples R China

2.Heilongjiang Acad Agr Sci, Pratacultural Res Inst, Harbin 150000, Peoples R China

关键词： Cercospora sojina Hara stress; DNA methylation; MASP; Homology analysis

期刊名称：PAKISTAN JOURNAL OF BOTANY （ 影响因子：0.9； 五年影响因子：1.0 ）

ISSN： 0556-3321

年卷期： 2024 年 56 卷 5 期

页码：

收录情况： SCI

摘要： Epigenetic mechanisms significantly influence plant stress responses, yet their role in soybean ( Glycine max ) responses to stress triggered by Cercospora sojina Hara infection remains unexplored. In this study, soybean strains Longpin 09-85 and Yulong 09-521, with respective high susceptibility and high resistance to C. sojina Hara infection, were analysed for differences in infection -induced DNA methylation and changes in gene expression using the methylation-sensitive amplified polymorphism (MSAP) technique. Post -infection, both strains exhibited increased genomic DNA demethylation that was significantly more pronounced in the Longpin 09-85 strain, thereby reducing the Longpin 09-85:Yulong 09-521 DNA methylation ratio. Furthermore, the difference between stress -induced methylation and demethylation changes in DNA of the susceptible strain was significantly greater than that of the resistant strain, suggesting an important role for genomic DNA methylation in soybean resistance to stress induced by C. sojina Hara infection. Analysis of the total of 22 MSAP products that were differentially methylated between strains revealed four products that encoded proteins with homologies to proteins with known functions, as well as diverse types of methylation changes occurring in response to C. sojina Hara infectioninduced stress. These findings establish a foundation on which to build future investigations of molecular mechanisms governing soybean responses to C. sojina Hara-induced stress.