DNA Template Preparation and Technology System Building for AFLP Analysis of Chinese Cabbage (Brassica campestris L. ssp pekinensis)
文献类型： 外文期刊
作者： Meng, Shuchun 1 ; Zhang, Haiying 1 ; Liu, Ling 1 ; Liu, Yumei 2 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China
2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
关键词： Chinese cabbage (Brassica campestris L. ssp pekinensis);DNA isolation;AFLP;silver-staining
期刊名称：INTERNATIONAL SYMPOSIUM ON VEGETABLE PRODUCTION, QUALITY AND PROCESS STANDARDIZATION IN CHAIN: A WORLDWIDE PERSPECTIVE
ISSN： 0567-7572
年卷期： 2012 年 944 卷
页码：
收录情况： SCI
摘要： In this study, the improved method of CTAB was used to extract genomic DNA from tender leaves of Chinese cabbage (Brassica campestris L. ssp. pekinensis). DNA restriction reaction, ligase reaction, pre-amplification and selective amplification were optimized for establishment of AFLP reaction system and clear DNA fingerprints. The results indicated: (1) The effects of restriction of DNA, lingation of oligonucleotide adapters as well as amplification should be affected by the quality of genomic DNA. Clear finger map were obtained by the improved CTAB method which is suitable for AFLP analysis on Chinese cabbage. (2) There were 150 ng DNA template, EcoRI 1.25U, MseI 1.25U, 5xReaction Buffer, in the best 12.5 mu l system of restriction at 37 degrees C for 4 h. The optimum temperature in the lingation system was 20 degrees C for 2.5 h. (3) The clear and stable amplification patterns could be obtained from six Primer pairs (E-AAC/M-CAG, E-AAG/M-CAC, E-ACA/M-CTG, E-ACT/M-CAC, E-ACT/M-CTT, E-ACT/M-CTC). Based on the silver-staining AFLP system, this paper might provide a method for the further study in the field of molecular marker in genetic diversity, new cultivars breeding and genetic relationship in Chinese cabbage.
- 相关文献
作者其他论文 更多>>
-
A natural variant of NON-RIPENING promotes fruit ripening in watermelon
作者：Wang, Jinfang;Yu, Yongtao;Guo, Shaogui;Zhang, Jie;Ren, Yi;Tian, Shouwei;Li, Maoying;Liao, Shengjin;Gong, Guoyi;Zhang, Haiying;Xu, Yong
关键词：
-
The NAC transcription factor ClNAC100 positively regulates plant height and fruit size in watermelon
作者：Wang, Jinfang;Zhu, Yi;Li, Maoying;Zhang, Haiying;Zhang, Xilong;Tian, Shouwei;Ren, Yi;Yu, Yongtao;Liao, Shengjin;Gong, Guoyi;Xu, Yong;Zhang, Jie;Zhu, Yi;Velkov, Nikolay
关键词：NAC transcription factor; fruit size; gibberellin; watermelon
-
Metabolome insights into nutrients and glucosinolates in broccoli and lacinato kale
作者：Gao, Wenzheng;Liu, Yumei;Han, Fengqing;Zhang, Shuo;Li, Zhansheng;Lai, Shangxiang;Liu, Guangmin;He, Hongju
关键词：Leaves; By-product; UPLC-MS/MS; HPLC; Plant chassis
-
One-step creation of CMS lines using a BoCENH3-based haploid induction system in Brassica crop
作者：Han, Fengqing;Liu, Yuxiang;Liu, Yumei;Li, Zhansheng;Zhang, Xiaoli;Liu, Yuxiang;Zhao, Hong
关键词：
-
Editing eIF4E in the Watermelon Genome Using CRISPR/Cas9 Technology Confers Resistance to ZYMV
作者：Li, Maoying;Qiu, Yanhong;Zhu, Dongyang;Xu, Xiulan;Tian, Shouwei;Wang, Jinfang;Yu, Yongtao;Ren, Yi;Gong, Guoyi;Zhang, Haiying;Xu, Yong;Zhang, Jie;Zhu, Dongyang
关键词：watermelon; eukaryotic translation initiation factor 4E (eIF4E); CRISPR/Cas9
-
Recurrent excision of a hAT-like transposable element in CmAPRR2 leads to the "shooting star" melon phenotype
作者：Zhang, Wei;Shen, Huolin;Xu, Yong;Zhang, Wei;Liao, Shengjin;Zhang, Jie;Sun, Honghe;Li, Shaofang;Zhang, Haiying;Gong, Guoyi;Xu, Yong
关键词：shooting star; chlorophyll accumulation; hAT; transposon; melon
-
Identification of allelic relationship and translocation region among chromosomal translocation lines that leads to less-seed watermelon
作者：Jiao, Di;Anees, Muhammad;Zhu, Hongju;Liu, Wenge;Jiao, Di;Zhao, Hong;Zhang, Jie;Zhang, Haiying;Gong, Guoyi;Xu, Yong;Sun, Honghe;Sun, Honghe
关键词：
