文献类型： 外文期刊

作者： Wu Limin ¹ ; Yang Tianyu ² ; Dong Guojun ³ ; He Jihong ² ; Dong Kongjun ² ; Wang Huizhong ¹ ; Yu Yanchun ¹ ;

作者机构： 1.Hangzhou Normal Univ, Coll Life Sci, Hangzhou 310036, Zhejiang, Peoples R China

2.Gansu Acad Agr Sci, Crop Res Inst, Lanzhou 730070, Peoples R China

3.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China

关键词： Proso millet;Temperature;Medium;Genotype;Carbohydrate

期刊名称：ADVANCES IN BROOMCORN MILLET RESEARCH, PROCEEDINGS OF THE 1ST INTERNATIONAL SYMPOSIUM ON BROOMCORN MILLET, 2012

ISSN：

年卷期： 2012 年

页码：

收录情况： SCI

摘要： Proso millet is a promising species for rich nutrition, drought and barren-tolerance. Influences factors on anther-derived callus induction efficiency were investigated when microspores of tested anthers at the late-uninucleate to early-binucleate stage were used as explants. The results indicated the callus induction was strongly affected by genotype. Several highly responsive cultivars of proso millet with desired traits had been identified and they could be directly used in crop improvement by breeding. Cold pre-treatment for three weeks of spikes then 32.5 degrees C shortly heat post-incubation culture could significant improve callus yield. Moreover, W14 basal medium and combination 60 g/L sucrose with 60 g/L maltose as carbon source were optimal for anther culture of proso millet in this study. To our best knowledge, this is the first report on successful induction of pollen-derived callus in pros() millet and it will accelerate the breeding programs.