文献类型： 会议论文

第一作者： Wu Limin

作者： Wu Limin ¹ ; Yang Tianyu ² ; Dong Guojun ³ ; He Jihong ¹ ; Dong Kongjun ² ; Wang Huizhong ⁴ ; Yu Yanchun ⁴ ;

作者机构： 1.College of Life Science, Hangzhou Normal University, Hangzhou 310036, China

2.Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China

3.State Key Laboratory of Rice Biology, China National Rice Research Institute Hangzhou,Zhejiang, 310006, China

4.State Key Laboratory of Rice Biology, China National Rice Research Institute Hangzhou, Zhejiang, 310006, China

关键词： Proso millet;Temperature;Medium;Genotype;Carbohydrate

会议名称： International Symposium on Broomcorn Millet

主办单位：

页码： 125-135

摘要： Proso millet is a promising species for rich nutrition, drought and barren-tolerance. Influences factors on anther-derived callus induction efficiency were investigated when microspores of tested anthers at the late-uninucleate to early-binucleate stage were used as explants. The results indicated the callus induction was strongly affected by genotype. Several highly responsive cultivars of proso millet with desired traits had been identified and they could be directly used in crop improvement by breeding. Cold pre-treatment for three weeks of spikes then 32.5 °C shortly heat post-incubation culture could significant improve callus yield. Moreover, W14 basal medium and combination 60 g/L sucrose with 60 g/L maltose as carbon source were optimal foranther culture of proso millet in this study. To our best knowledge, this is the first report on successful induction of pollen-derived callus in proso millet and it will accelerate the breeding programs.

分类号： S516-532