文献类型： 外文期刊

作者： Ren Hong-yan ¹ ; Zheng Xin-min ² ; Chen Hong-xing ³ ; Li Kui ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Key Lab Farm Anim Genet Resources & Utilizat, Inspect Test Ctr Biosafety Genetically Modified A, Minist Agr,Inst Anim Sci, Beijing 100193, Peoples R China

2.Hubei Acad Agr Sci, Key Lab Anim Embryo & Mol Breeding Hubei Prov, Inst Vet & Anim Sci, Wuhan 430064, Peoples R China

3.Beijing Inst Biotechnol, Beijing 100071, Peoples R China

关键词： transgenic pigs;sFat-1 gene;omega-3 polyunsaturated fatty acids;microinjection

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2011 年 10 卷 10 期

页码：

收录情况： SCI

摘要： Polyunsaturated fatty acids (PUFAs) are essential for normal growth in mammals, especially the omega-3 PUFAs, which play important roles in preventing several life-threatening diseases, such as coronary heart disease and diabetes. In this study, we aimed to investigate whether the sFat-1 gene from Caenorhabditis briggsae could be functionally expressed in transgenic pigs, and whether the transgenic could synthesize high quality omega-3 PUFAs endogenously. In this study, a gene construct consisting of CMV promoter and 1.9 kb cDNA of omega-3 fatty acid desaturase gene (sFat-1) from C. briggsae was injected into the male pronucleus of pig embryos by microinjection. The piglets were screened for the transgene by PCR, Southern blot and reverse transcription-PCR analysis. Pigs that give positive results were mated with wild-type pigs to produce the next generation and the transmission of transgene was examined by PCR analysis. Fatty acids compositions of various tissues in the transgenic pigs were then analyzed by gas chromatograph. A total of 878 embryos were transferred into 42 recipients, among which 29 successfully got pregnant and gave birth to a total of 162 piglets, and 8 of them were identified to be transgenic. Fatty acid compositions in the transgenic pigs were altered, and the levels of omega-6: omega-3 ratios were decreased from 14.53 in the control to 2.62 in Fat-1 transgenic pigs. A number of primary sFat-1-transgenic pigs were bred in this study, which lays the foundation for cultivation of new varieties of transgenic pigs.