文献类型： 外文期刊

作者： Shen, Jian-Shuang ¹ ; Lan, Lan ² ; Kan, Sheng-Long ² ; Cheng, He-Feng ¹ ; Peng, Dan ² ; Wan, Zi-Yun ¹ ; Hu, Yue ¹ ; Huang, Xiao-Ling ¹ ; Li, Xue-Qin ¹ ; Ye, Yuan-Jun ⁵ ; Tembrock, Luke R. ⁶ ; Wu, Zhi-Qiang ² ; Jin, Song-Heng ¹ ;

作者机构： 1.Zhejiang A&F Univ, Jiyang Coll, Zhuji 311800, Zhejiang, Peoples R China

2.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Guangdong Lab Lingnan Modern Agr, Shenzhen Branch,Genome Anal Lab,Minist Agr & Rural, Shenzhen 518120, Peoples R China

3.Murdoch Univ, Sch Med Mol & Forens Sci, 90 South St, Perth, WA, Australia

4.Chinese Acad Agr Sci, Kunpeng Inst Modern Agr Foshan, Agr Genom Inst Shenzhen, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr, Foshan 528200, Guangdong, Peoples R China

5.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangdong Key Lab Ornamental Plant Germplasm Innov, Guangzhou 510640, Peoples R China

6.Colorado State Univ, Dept Agr Biol, Ft Collins, CO 80523 USA

7.Huzhou Coll, Dept Life Sci & Hlth, Huzhou 313000, Zhejiang, Peoples R China

关键词： Ericaceae; genome evolution; heat shock proteins; horticulture; pan-genome

期刊名称：JOURNAL OF SYSTEMATICS AND EVOLUTION （ 影响因子：3.7； 五年影响因子：3.8 ）

ISSN： 1674-4918

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Rhododendron is the largest genus in Ericaceae and is well known for its diversity and beauty of flowers present in different species, making it a much-revered lineage of ornamental plants. Many species of Rhododendron are intolerant of high temperatures, which are becoming more common and intense in urban areas under global climate change. Therefore, the discovery and description of genes from heattolerant Rhododendron lineages are essential in the development of new climate-resilient cultivars. One such species known to be heat tolerant is Rhododendron x pulchrum Sweet. To better understand the genomics of heat tolerance in this species, we assembled a haplotype- resolved and chromosome- scale genome for R. x pulchrum, which had a genome size of 509 Mb; a scaffold N50 of 37 251 370 bp; and contained 35 610 genes. In addition, based on the same reannotation pipeline, we conducted pan-genomic analyses for all seven available chromosome- scale Rhododendron genomes and found 14 415 gene groups shared across all species and 18 018 gene groups distributed in the other species, including 1879 gene groups found in only a single species. Finally, we analyzed the transcriptomic data from heat- treated and non-heat-treated R. x pulchrum plants to quantify the genes that are most important during heat stress in an effort to inform the development of climate-resilient cultivars. This study provides insight into the genome diversity in Rhododendron and targets several genes related to agronomic traits that may help in further analysis.