文献类型： 外文期刊

作者： Shi, Yuan ¹ ; Wang, Yan ¹ ; Pan, Yaowen ¹ ; Deng, Changhao ¹ ; Zeng, Ting ¹ ; Su, Deding ¹ ; Lu, Wang ¹ ; Lin, Yuxiang ¹ ; Han, Junnan ¹ ; Deng, Wei ¹ ; Wu, Shuang ³ ; Liu, Yudong ⁴ ; Li, Ning ⁵ ; Li, Ji ⁶ ; Dong, Biao ⁶ ; Abid, Ghassen ⁷ ; Bouzayen, Mondher ² ; Pirrello, Julien ⁸ ; Li, Zhengguo ¹ ; Huang, Baowen ¹ ;

作者机构： 1.Chongqing Univ, Sch Life Sci, Key Lab Plant Hormones Regulat & Mol Breeding Chon, Chongqing 401331, Peoples R China

2.Chongqing Univ, Inst Adv Interdisciplinary Studies, Ctr Plant Funct Genom & Synthet Biol, Chongqing 401331, Peoples R China

3.Fujian Agr & Forestry Univ, Haixia Inst Sci & Technol, Coll Hort, Fuzhou 350002, Peoples R China

4.Zhengzhou Univ, Sch Agr Sci, Zhengzhou 450001, Peoples R China

5.Xinjiang Acad Agr Sci, Inst Hort Crops, Key Lab Genome Res & Genet Improvement Xinjiang Ch, Urumqi, Peoples R China

6.Nanjing Agr Univ, Coll Hort, Natl Key Lab Crop Genet & Germplasm Enhancement &, Nanjing 210095, Peoples R China

7.Ctr Biotechnol Borj Cedria, Lab Legumes & Sustainable Agrosyst, PB 901, Hammam Lif 2050, Tunisia

8.Univ Toulouse, UPS, Lab Rech Sci Vegetales Genom & Biotechnol Fruits, Toulouse INP,CNRS,UMR5546, Toulouse, France

关键词： trichome formation; tomato; type VI glandular trichome; terpenoid accumulation; spider mites; aphids

期刊名称：PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：7.0 ）

ISSN： 0960-7412

年卷期： 2025 年 122 卷 3 期

页码：

收录情况： SCI

摘要： Trichomes of aerial plant organs contribute to adaptive responses to abiotic and biotic stresses. In horticultural plants, increasing glandular trichome density is an effective breeding strategy to enhance resistance to herbivores through promoting the capacity to produce specialized metabolites. The regulatory mechanisms controlling multicellular trichome formation are only partially understood. In this study, we reveal that SlGRAS9 and SlMYC1 transcription factors form a regulatory module controlling glandular trichome formation in multiple tissues. Knockout of SlGRAS9 or overexpression of SlMYC1 in tomato leads to an increased number of type VI glandular trichomes and to higher terpenoid accumulation in leaves, petals, sepals, and fruits. Conversely, knockout of SlMYC1 results in reduced type VI glandular trichomes number and terpenoid levels. Promoter-binding and genetic interaction experiments revealed that SlGRAS9 negatively regulates the transcription of SlMYC1, indicating that the regulation of glandular trichome formation by SlGRAS9 is dependent, at least partly, on SlMYC1. Consistently, both SlGRAS9 knockout and SlMYC1 overexpression result in higher tolerance of tomato plants to spider mites and aphids. In addition to adding some of the missing components to the mechanisms controlling formation of type VI glandular trichome, our findings also uncover new targets for breeding strategies aimed at improving crop protection against pest invasion, thus ensuring crop yield resilience to climate change.