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Development and application of whole-chromosome painting of chromosomes 7A and 8A of Arachis duranensis based on chromosome-specific single-copy oligonucleotides

文献类型： 外文期刊

作者： Li, Chenyu 1 ; Fu, Liuyang 2 ; Wang, Qian 2 ; Liu, Hua 3 ; Chen, Guoquan 2 ; Qi, Feiyan 3 ; Zhang, Maoning 1 ; Jia, Yaoguang 2 ; Li, Xiaona 2 ; Huang, Bingyan 3 ; Dong, Wenzhao 3 ; Du, Pei 3 ; Zhang, Xinyou 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Agr, Nanjing 210095, Jiangsu, Peoples R China

2.Henan Agr Univ, Coll Life Sci, Zhengzhou 450001, Henan, Peoples R China

3.Henan Acad Agr Sci, Inst Crop Mol Breeding, Nation Ind Innovat Ctr Biobreeding, Shennong Lab,Key Lab Oil Crops Huang Huai Hai Plai, Zhengzhou 450002, Henan, Peoples R China

关键词： Arachis species; chromosome painting; single-copy oligonucleotide; chromosomal variation; interspecific hybridization

期刊名称：GENOME （ 影响因子：3.1； 五年影响因子：2.6 ）

ISSN： 0831-2796

年卷期： 2024 年 67 卷 6 期

页码：

收录情况： SCI

摘要： For peanut, the lack of stable cytological markers is a barrier to tracking specific chromosomes, elucidating the genetic relationships between genomes and identifying chromosomal variations. Chromosome mapping using single-copy oligonucleotide (oligo) probe libraries has unique advantages for identifying homologous chromosomes and chromosomal rearrangements. In this study, we developed two whole-chromosome single-copy oligo probe libraries, LS-7A and LS-8A, based on the reference genome sequences of chromosomes 7A and 8A of Arachis duranensis. Fluorescence in situ hybridization (FISH) analysis confirmed that the libraries could specifically paint chromosomes 7 and 8. In addition, sequential FISH and electronic localization of LS-7A and LS-8A in A. duranensis (AA) and A. ipaensis (BB) showed that chromosomes 7A and 8A contained translocations and inversions relative to chromosomes 7B and 8B. Analysis of the chromosomes of wild Arachis species using LS-8A confirmed that this library could accurately and effectively identify A genome species. Finally, LS-7A and LS-8A were used to paint the chromosomes of interspecific hybrids and their progenies, which verified the authenticity of the interspecific hybrids and identified a disomic addition line. This study provides a model for developing specific oligo probes to identify the structural variations of other chromosomes in Arachis and demonstrates the practical utility of LS-7A and LS-8A.

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