Inter-laboratory validation by event-specific qPCR methods for the detection of genetically modified insect and herbicide-tolerant maize DBN9501
文献类型： 外文期刊
作者： Wang, Jing 1 ; Xiao, Bing 2 ; Zhang, Ruiying 1 ; Guan, Haitao 1 ; Yang, Yang 1 ; Ding, Yijia 1 ; Yuan, Ran 1 ; Zhang, Xiaolei 1 ; Li, Lingyan 4 ; Liang, Jingang 5 ; Wen, Hongtao 1 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Qual & Safety Inst Agr Prod, Harbin 150086, Peoples R China
2.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China
3.China Agr Univ, Coll Resources & Environm Sci, Beijing 100091, Peoples R China
4.Beijing Vocat Coll Agr, Beijing 102442, Peoples R China
5.Minist Agr & Rural Affairs Peoples Republ China, Dev Ctr Sci & Technol, Beijing 100176, Peoples R China
关键词： Genetically modified maize; DBN9501; GMO; Event-specific method; Inter-laboratory ring trial; RT-PCR
期刊名称：JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY （ 影响因子：2.4； 五年影响因子：1.5 ）
ISSN： 1661-5751
年卷期： 2023 年
页码：
收录情况： SCI
摘要： DBN9501 is a new maize transgenic event characterised by being resistance to insects and herbicides. To meet genetically modified (GM)-labeling requirements and monitor the unintended release of genetically modified organisms (GMOs), developing a creditable and applicable method for identifying and quantifying GM events is essential. Herein we developed an event-specific method and validated it through inter-laboratory ring trials using blind samples. The limit of detection (LOD) and limit of quantification (LOQ) of copy number ratio were confirmed at 0.05% and 0.1%, respectively. The quantitative bias ranged from -3.52 to 10.38%, and the relative standard deviation (RSD) of the method was < 25%. Furthermore, the expanded uncertainty for the blind samples S1 - S5 was 0.22%, 0.10%, 0.05%, 0.03%, and 0.02%. These results demonstrated that the event-specific quantitative PCR method could identify and quantify GM DBN9501 for further routine lab analysis.
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