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OsNAR2.1 induced endogenous nitrogen concentration variation affects transcriptional expression of miRNAs in rice

文献类型： 外文期刊

作者： Zhang, Yong 1 ; Fan, Xiaoru 2 ; Wang, Yulong 3 ; Kong, Pulin 3 ; Zhao, Ling 1 ; Fan, Xiaorong 3 ; Zhang, Yadong 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Crops, Jiangsu High Qual Rice Res & Dev Ctr, Nanjing Branch,China Natl Ctr Rice Improvement, Nanjing, Peoples R China

2.Anshan Normal Univ, Sch Chem & Life Sci, Anshan, Peoples R China

3.Nanjing Agr Univ, Coll Resources & Environm Sci, State Key Lab Crop Genet & Germplasm Enhancement, Key Lab Plant Nutr & Fertilizat Low Middle Reaches, Nanjing, Peoples R China

4.Zhongshan Biol Breeding Lab, Nanjing, Peoples R China

关键词： nitrogen concentration; OsNAR2; 1; miRNA; rice; previously unidentified miRNA

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： The studies of rice nitrogen concentration on the expression of miRNA so far are mostly limited to the exogenous nitrogen, leaving the effect of endogenous nitrogen largely unexplored. OsNAR2.1 is a high-affinity nitrate transporter partner protein which plays a central role in nitrate absorption and translocation in rice. The expression of OsNAR2.1 could influence the concentration of the endogenous nitrogen in rice. We showed that the expression and production of miRNA in rice can be influenced by manipulating the endogenous nitrogen concentration via OsNAR2.1 transgenic lines. The small RNA content, particularly 24 nucleotides small RNA, expressed differently in two transgenic rice lines (nitrogen efficient line with overexpression of OsNAR2.1 (Ov199), nitrogen-inefficient line with knockdown OsNAR2.1 by RNAi (RNAi)) compared to the wild-type (NP). Comparative hierarchical clustering expression pattern analysis revealed that the expression profiles of mature miRNA in both transgenic lines were different from NP. Several previously unidentified miRNAs were identified to be differentially expressed under different nitrogen concentrations, namely miR1874, miR5150, chr3-36147, chr4-27017 and chr5-21745. In conclusion, our findings suggest that the level of endogenous nitrogen concentration variation by overexpression or knockdown OsNAR2.1 could mediate the expression pattern and intensity of miRNA in rice, which is of high potential to be used in molecular breeding to improve the rice responses towards nitrogen utilization.

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