您好，欢迎访问天津市农业科学院 机构知识库！
天津市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Precise genome editing of Dense and Erect Panicle 1 promotes rice sheath blight resistance and yield production in japonica rice

文献类型： 外文期刊

作者： Zhu, Hongyao 1 ; Zhou, Tiange 1 ; Guan, Jiaming 4 ; Li, Zhuo 1 ; Yang, Xiurong 5 ; Li, Yuejiao 5 ; Sun, Jian 6 ; Xu, Quan 6 ; Xuan, Yuan Hu 1 ;

作者机构： 1.Nankai Univ, Natl Pesticide Engn Res Ctr Tianjin, State Key Lab Elementoorgan Chem, Tianjin, Peoples R China

2.Nankai Univ, Natl Pesticide Engn Res Ctr Tianjin, Dept Plant Protect, Tianjin, Peoples R China

3.Shenyang Agr Univ, Coll Plant Protect, Shenyang, Peoples R China

4.Xianghu Lab, Hangzhou, Peoples R China

5.Tianjin Acad Agr Sci, Inst Plant Protect, Tianjin, Peoples R China

6.Shenyang Agr Univ, Rice Res Inst, Shenyang, Peoples R China

7.Yazhouwan Natl Lab, Sanya, Hainan, Peoples R China

关键词： DEP1; genome editing; yield; sheath blight; resistance; rice

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.5； 五年影响因子：12.4 ）

ISSN： 1467-7644

年卷期： 2025 年 23 卷 5 期

页码：

收录情况： SCI

摘要： The primary goals of crop breeding are to enhance yield and improve disease resistance. However, the "trade-off" mechanism, in which signalling pathways for resistance and yield are antagonistically regulated, poses challenges for achieving both simultaneously. Previously, we demonstrated that knock-out mutants of the Dense and Erect Panicle 1 (DEP1) gene can significantly enhance rice resistance to sheath blight (ShB), and we mapped DEP1's association with panicle length. In this study, we discovered that dep1 mutants significantly reduced rice yield. Nonetheless, truncated DEP1 was able to achieve both ShB resistance and yield increase in japonica rice. To further explore the function of truncated DEP1 in promoting yield and ShB resistance, we generated CRISPR/Cas9-mediated genome editing mutants, including a full-length deletion mutant of DEP1, named dep1, and a truncated version, dep1-cys. Upon inoculation with Rhizoctonia solani, the dep1-cys mutant demonstrated stronger ShB resistance than the dep1 mutant. Additionally, dep1-cys increased yield per plant, whereas dep1 reduced it. Compared to the full DEP1 protein, the truncated DEP1 (dep1-cys) demonstrated a decreased interaction affinity with IDD14 and increased affinity with IDD10, which are known to positively and negatively regulate ShB resistance through the activation of PIN1a and ETR2, respectively. The dep1-cys mutant exhibited higher PIN1a and lower ETR2 expression than wild-type plants, suggesting that dep1-cys modulated IDD14 and IDD10 interactions to regulate PIN1a and ETR2, thereby enhancing ShB resistance. Overall, these data indicate that precise genome editing of DEP1 could simultaneously improve both ShB resistance and yield, effectively mitigating trade-off regulation in rice.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：天津市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台