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Reconstruction of the violacein biosynthetic pathway from Duganella sp B2 in different heterologous hosts

文献类型： 外文期刊

作者： Jiang, Pei-xia 1 ; Wang, Hai-sheng 2 ; Zhang, Chong 1 ; Lou, Kai 3 ; Xing, Xin-Hui 1 ;

作者机构： 1.Tsinghua Univ, Inst Biochem Engn, Dept Chem Engn, Beijing 100084, Peoples R China

2.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China

3.Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi 830091, Peoples R China

关键词： Violacein;Deoxyviolacein;Biosynthesis;Duganella sp B2;Heterologous expression

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2010 年 86 卷 4 期

页码：

收录情况： SCI

摘要： Violacein is a bacteria-originated indolocarbazole pigment with potential applications due to its various bioactivities such as anti-tumor, antiviral, and antifungal activities. However, stable mass production of this pigment is difficult due to its low productivities and the instability of wild-type violacein-producing strains. In order to establish a stable and efficient production system for violacein, the violacein synthesis pathway from a new species of Duganella sp. B2 was reconstructed in different bacterial strains including Escherichia coli, Citrobacter freundii, and Enterobacter aerogenes by using different vectors. The gene cluster that encodes five enzymes involved in the violacein biosynthetic pathway was first isolated from Duganella sp. B2, and three recombinant expression vectors were constructed using the T7 promoter or the alkane-responsive promoter PalkB. Our results showed that violacein could be stably synthesized in E. coli, C. freundii, and E. aerogenes. Interestingly, we found that there were great differences between the different recombinant strains, not only in the protein expression profiles pertaining to violacein biosynthesis but also in the productivity and composition of crude violacein. Among the host strains tested, the crude violacein production by the recombinant C. freundii strain reached 1.68 g L(-1) in shake flask cultures, which was 4-fold higher than the highest production previously reported in flask culture by other groups. To the best of our knowledge, this is the first report on the efficient production of violacein by genetically engineered strains.

  • 相关文献

[1]Pathway redesign for deoxyviolacein biosynthesis in Citrobacter freundii and characterization of this pigment. Jiang, Pei-xia,Xiao, Su,Fang, Ming-yue,Zhang, Rui-ping,Xing, Xin-Hui,Jiang, Pei-xia,Wang, Hai-sheng,He, Shu-ying,Lou, Kai. 2012

[2]Optimization of culture conditions for violacein production by a new strain of Duganella sp B2. Jiang, Peixia,Lu, Yuan,Xing, Xin-Hui,Wang, Haisheng,Ruan, Zhiyong,Jiang, Ruibo,Lou, Kai,Wei, Dong. 2009

[3]Fed-batch fermentation of recombinant Citrobacter freundii with expression of a violacein-synthesizing gene cluster for efficient violacein production from glycerol. Yang, Cheng,Jiang, Peixia,Xiao, Su,Zhang, Chong,Xing, Xin-Hui,Lou, Kai.

[4]Production of violet pigment by a newly isolated psychrotrophic bacterium from a glacier in Xinjiang, China. Lu, Yuan,Wang, Liyan,Xue, Yuan,Zhang, Chong,Xing, Xin-Hui,Lou, Kai,Zhang, Zhidong,Li, Yong,Zhang, Guifeng,Bi, Jingxiu,Su, Zhiguo.

[5]High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. Fang, Ming-Yue,Zhang, Chong,Xing, Xin-Hui,Yang, Song,Cui, Jin-Yu,Jiang, Pei-Xia,Lou, Kai,Wachi, Masaaki. 2015

[6]Association Analysis of the nced and rab28 Genes with Phenotypic Traits Under Water Stress in Maize. Su, Zhijun,Li, Xinhai,Hao, Zhuanfang,Xie, Chuanxiao,Li, Mingshun,Weng, Jianfeng,Zhang, Degui,Zhang, Shihuang,Su, Zhijun,Li, Xinhai,Hao, Zhuanfang,Xie, Chuanxiao,Li, Mingshun,Weng, Jianfeng,Zhang, Degui,Zhang, Shihuang,Liang, Xiaoling,Su, Zhijun,Wang, Zhigang,Gao, Julin.

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