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A novel functional allele of Ehd3 controls flowering time in rice

文献类型： 外文期刊

作者： Hong, Zhipeng 1 ; Liu, Yingxiang 1 ; He, Mingliang 1 ; Zhou, Wenyan 2 ; Sui, Jingjing 2 ; Tian, Xiaojie 1 ; Guan, Qingjie 2 ; Yu, Xinglong 3 ; Li, Kun 4 ; Bu, Qingyun 1 ; Li, Xiufeng 1 ;

作者机构： 1.Chinese Acad Sci, Northeast Inst Geog & Agroecol, State Key Lab Black Soils Conservat & Utilizat, Key Lab Soybean Mol Design Breeding, Harbin 150081, Peoples R China

2.Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China

3.Beidahuang Grp Erdaohe Farm CO LTD, Harbin, Peoples R China

4.Heilongjiang Acad Agr Sci, Biotechnol Inst, Harbin 150080, Peoples R China

5.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

关键词： Rice; Heading date; Haplotype; Ehd3

期刊名称：MOLECULAR BREEDING （ 影响因子：3.1； 五年影响因子：3.1 ）

ISSN： 1380-3743

年卷期： 2024 年 44 卷 5 期

页码：

收录情况： SCI

摘要： Rice flowering time determines its geographical distribution and yield traits. As a short-day plant, rice can grow in the northern long-day conditions due to the functional mutations of many photosensitive genes. In this study, to identify novel genes or alleles that regulate flowering time in high latitude region, two cultivar, Dongnong 413 (DN413) and Yukimochi (XN) showing extreme early flowering were used for investigation. DN413 is around 4.0 days earlier than XN, and both cultivars can be grown in II (2500 degree celsius-2700 degree celsius) to III (2300 degree celsius-2500 degree celsius) accumulated temperature zones. We found that the two cultivars shared the same genotype of heading date genes, including Hd1/2/4/5/6/16/17/18, Ehd2, DTH2, SE5, Hd3a. Importantly, a novel Ehd3 allele characterized by a A1146C substitution was identified, which results in the E382D substitution, hereafter the 382 position E is defined as Hap_E and the 382 position D is defined as Hap_D. Association analysis showed that Hap_E is earlier flowering than Hap_D. Subsequently, we construct DN413 Hap_D line by three times back-crossing DN413 with XN, and found the heading date of DN413 Hap_D was 1.7-3.5 days later than DN413. Moreover, Hap_E and Hap_D of Ehd3 were transformed into ehd3 mutant, respectively, and the Ehd3pro:Ehd3D/ehd3 flowered later than that Ehd3pro:Ehd3E/ehd3 by around 4.3 days. Furthermore, we showed Ehd3 functions as a transcriptional suppressor and the substitution of Asp-382 lost the inhibition activity in protoplasts. Finally, a CAPS marker was developed and used for genotyping and marker assistant breeding. Collectively, we discovered a novel functional allele of Ehd3, which can used as a valuable breeding target.

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