The role and molecular mechanism of flgK gene in biological properties, pathogenicity and virulence genes expression of Aeromonas hydrophila
文献类型： 外文期刊
作者： Lu, Jiahui 1 ; Xiong, Chuanyu 1 ; Wei, Jinming 1 ; Xiong, Caijiang 1 ; Long, Rui 1 ; Yu, Yongxiang 3 ; Ye, Hua 1 ; Ozdemir, Eda 4 ; Li, Yun 1 ; Wu, Ronghua 1 ;
作者机构： 1.Southwest Univ, Integrat Sci Ctr Germplasm Creat Western China CHO, Chongqing 400715, Peoples R China
2.Southwest Univ, Coll Fisheries, Aquaculture Engn Technol Res Ctr, Chongqing 400715, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
4.Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA
5.Southwest Univ, Coll Agron & Biotechnol, 2 Tiansheng Rd, Chongqing 400715, Peoples R China
关键词： Aeromonas hydrophila; FlgK gene; Biological properties; Pathogenicity; Virulence related genes
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 2022影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2024 年 258 卷
收录情况： SCI
摘要： Aeromonas hydrophila is a highly pathogenic aquatic resident bacterium that can cause co-morbidity in aquatic animals, waterfowl, poultry, and humans. Flagellum is the motility organ of bacteria important for bacterium tissue colonization and invasion. The flgK gene encodes a flagellar hook protein essential for normal flagellar formation. In order to explore the role of flgK in A. hydrophila, a flgK gene mutant strain of A. hydrophila (Delta flgKAH) was constructed using an efficient suicide plasmid-mediated homologous recombination method, and gene sequencing confirmed successful mutation of the flgK gene. The biological properties, pathogenicity and virulence genes expression were compared. The results showed that there was no significant difference in the growth, hemolytic, and swarming abilities, but the swimming and biofilm formation abilities of Delta flgK-AH were significantly reduced and the transmission electron microscope (TEM) results showed that the Delta flgK-AH strain did not have a flagellar structure. The median lethal dose (LD50) value of the Delta flgK-AH in Carassius auratus was 1.47-fold higher than that of the wild-type strain (WT-AH). The quantitative real-time PCR results showed that only the expression level of the lapA gene was up-regulated by 1.47 times compared with the WT-AH, while the expression levels of other genes were significantly down-regulated. In conclusion, flgK gene mutant led to a decline in the pathogenicity possibly by reducing swimming and biofilm formation abilities, these biological properties might result from the down-regulated expression of flagellate and pilus-related genes.
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