文献类型： 外文期刊

作者： Fan, Shu-Ting ¹ ; Zheng, Zi-Jing ¹ ; Feng, Qing ³ ; Zhu, Guan-Heng ¹ ;

作者机构： 1.Shenzhen Campus Sun Yat sen Univ, Sch Agr & Biotechnol, Shenzhen 518107, Guangdong, Peoples R China

2.Sun Yat sen Univ, Sch Agr & Biotechnol, State Key Lab Biocontrol, Shenzhen 518107, Peoples R China

3.Hainan Acad Agr Sci, Inst Plant Protect, Res Ctr Qual Safety & Stand Agr Prod, Haikou 571100, Hainan, Peoples R China

关键词： azadirachtin; apoptosis; midgut; fatbody; pesticides

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 35 期

页码：

收录情况： SCI

摘要： Azadirachtin is a widely used botanical pesticide for agricultural pest control worldwide. However, the molecular mechanisms of azadirachtin in insects are not fully understood. In this study, histological analysis and RNA sequencing were conducted to investigate the impact of azadirachtin on the larval development of Spodoptera frugiperda. Under azadirachtin exposure, the development was completely inhibited, and the major internal tissues, fat body, and midgut were strongly damaged under histological analysis. Differential gene expression analysis demonstrated that nutrient absorption and detoxification metabolism-related genes are differentially expressed. Interestingly, the expression of the apoptosis-related gene, caspase-8, was significantly inhibited under exposure to azadirachtin. In addition, after knocking down the expression of the caspase-8 gene, the fat body displayed a similar apoptotic phenotype as azadirachtin treatment; the distribution of chromatin and lipid droplets was uneven in the fat body cells. Thus, the results in this study demonstrated that exposure to azadirachtin rapidly activates apoptosis, resulting in innate tissue disruption, ultimately arresting larval development in S. frugiperda.