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Optimization of culture conditions for violacein production by a new strain of Duganella sp B2

文献类型： 外文期刊

作者： Wang, Haisheng 2 ; Jiang, Peixia 1 ; Lu, Yuan 1 ; Ruan, Zhiyong 3 ; Jiang, Ruibo 3 ; Xing, Xin-Hui 1 ; Lou, Kai 4 ; Wei, 1 ;

作者机构： 1.Tsinghua Univ, Inst Biochem Engn, Dept Chem Engn, Beijing 100084, Peoples R China

2.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China

3.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China

4.Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi 830000, Peoples R China

关键词： Response surface analysis;Optimization;Violacein;Duganella sp B2;Fermentation;Biosynthesis

期刊名称：BIOCHEMICAL ENGINEERING JOURNAL （ 影响因子：3.978； 五年影响因子：4.084 ）

ISSN： 1369-703X

年卷期： 2009 年 44 卷 2-3 期

页码：

收录情况： SCI

摘要： Duganella sp. B2 isolated from Xingjiang Municipality of China was found to be a novel violacein-producing bacterium. In this study, culture variables affecting violacein production were optimized statistically in shake flask experiments. The medium pH, culture volume, and medium components of potassium nitrate, L-tryptophan, and beef extract were identified by two-level Plackett-Burman design as the most significant factors for violacein production. The path of steepest ascent was undertaken to determine the optimal region of five significant factors. Box-Behnken design and response surface analysis were adopted to further investigate the interaction between the variables and determine the optimal values for violacein production. The optimal culture conditions for violacein production were: pH 6.71, potassium nitrate 1.18 g/L, ammonium ferrous sulfate 0.08 g/L, dipotassium hydrogen phosphate 0.25 g/L, magnesium sulfate 0.75 g/L, beef extract 1.53 g/L, L-tryptophan 0.74 g/L, soluble starch 13.0 g/L, 25 mL medium in a 250 mL flask, with an inoculum size of 10% (v/v). 1.62 g/L crude violacein was achieved using the optimized conditions after 32 h of cultivation. This proved to be much higher than any other violacein-producing strains so far reported. (C) 2008 Elsevier B.V. All rights reserved.

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