您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time

文献类型： 外文期刊

作者： Yuan, Yu-Xiang 1 ; Wu, Jian 1 ; Sun, Ri-Fei 1 ; Zhang, Xiao-Wei 2 ; Xu, Dong-Hui 1 ; Bonnema, Guusje 3 ; Wang, Xiao-Wu 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

2.Henan Acad Agr Sci, Inst Hort, Zhengzhou 450002, Peoples R China

3.Univ Wageningen & Res Ctr, Lab Plant Breeding, NL-6708 PB Wageningen, Netherlands

关键词： BrFLC1;flowering time;splicing pattern;splicing site mutation

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2009 年 60 卷 4 期

页码：

收录情况： SCI

摘要： FLOWERING LOCUS C (FLC), encoding a MADS-domain transcription factor in Arabidopsis, is a repressor of flowering involved in the vernalization pathway. This provides a good reference for Brassica species. Genomes of Brassica species contain several FLC homologues and several of these colocalize with flowering-time QTL. Here the analysis of sequence variation of BrFLC1 in Brassica rapa and its association with the flowering-time phenotype is reported. The analysis revealed that a G -> A polymorphism at the 5' splice site in intron 6 of BrFLC1 is associated with flowering phenotype. Three BrFLC1 alleles with alternative splicing patterns, including two with different parts of intron 6 retained and one with the entire exon 6 excluded from the transcript, were identified in addition to alleles with normal splicing. It was inferred that aberrant splicing of the pre-mRNA leads to loss-of-function of BrFLC1. A CAPS marker was developed for this locus to distinguish Pi6+1(G) and Pi6+1(A). The polymorphism detected with this marker was significantly associated with flowering time in a collection of 121 B. rapa accessions and in a segregating Chinese cabbage doubled-haploid population. These findings suggest that a naturally occurring splicing mutation in the BrFLC1 gene contributes greatly to flowering-time variation in B. rapa.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台