文献类型： 外文期刊

作者： Lou, Ping ¹ ; Kang, Jungen ² ; Zhang, Guoyu ² ; Bonnema, Guusje ² ; Fang, Zhiyuan ³ ; Wang, Xiaowu;

作者机构： 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China; Wageningen Univ, Lab Plant Breeding, NL-6700 AJ Wageningen, Netherlands; Shanxi Acad Agr Sci, Inst Vegetables, Shanxi 030031, Peoples R China; NW Sci Tech Univ Agr & Forestry, Coll Hort, Shanxi 712100, Peoples R China

3.Chinese Acad Agr Sci, Inst Vegetables &

关键词： Brassica oleracea;dominant genetic male sterility;near isogenic lines (NILs);expression profiling;transcript derived fragment (TDF);pectin methylesterase inhibitor (PMEI)

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2007 年 172 卷 1 期

页码：

收录情况： SCI

摘要： A spontaneous male-sterile mutation affecting anther development has been identified in cabbage line 79-399-3. The male sterility was associated with abnormal callose degeneration and failure of microspore separation during anther development. Three pairs near-isogenic lines (NILs) with or without the dominant male sterility allele (MS-cd1) were profiled by cDNA-AFLP to detect differentially expressed genes during flower bud development. Out of 6500 transcript derived fragments (TDFs) inspected, 27 were differentially expressed between fertile and male sterile plants in all three sets of NILs. All 27 TDFs were sequenced and BLAST searching revealed that 14 out of 27 fragments were highly homologous to genes with known or predicted function in Arabidopsis. Moreover, three of these TDFs were homologous to genes involved in cell wall formation and degradation, including plant invertase/pectin methylesterase inhibitor (PMEI) and polysaccharide lyase. RT-PCR experiments revealed that PMEI was expressed strongly in the anther and filament. Analysis of a set of NILs with different male sterility types showed that PMEI was also not expressed in a Nigra CMS NIL, a recessive male sterile NIL and an Ogura CMS NIL compared to their corresponding fertile NILs. Based on both cytological and transcriptional data we suggest that the dominant MS-cd1 mutant gene may disrupts proper separation of pollens from tetrads, which leads to the suppressed expression of a number of genes including BOPME11, a gene likely involved in the degradation of pectin. (c) 2006 Elsevier Ireland Ltd. All rights reserved.