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IsoSeq and RNA-Seq analyses uncover the molecular response of Dalbergia odorifera T. Chen to low temperature

文献类型： 外文期刊

作者： Li, Xiaowen 1 ; Yang, Sheng 1 ; Liu, Xing 1 ; Xia, Haitao 1 ; Chen, Qiuxia 1 ; Liu, Yu 1 ; Hong, Zhou 2 ; Wang, Jinwang 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Zhejiang Inst Subtrop Crops, Wenzhou 325005, Peoples R China

2.Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China

关键词： Cold acclimation; Cold tolerance; Dalbergia odorifera T; Chen; RNA-Seq; IsoSeq; Alternative splicing (AS)

期刊名称：GENE （ 影响因子：3.913； 五年影响因子：3.48 ）

ISSN： 0378-1119

年卷期： 2022 年 847 卷

页码：

收录情况： SCI

摘要： In previous studies, we found young plants propagated from acclimated D. odorifera T. Chen trees displayed increased cold tolerance. To understand the molecular and physiological mechanisms underlying this phenom-enon, we prepared acclimated and non-acclimated young D. odorifera T. Chen trees for physiological, RNA-Seq, and IsoSeq analyses. First, both the acclimated and non-acclimated young trees were grown in 10 degrees C condition, then treated at-3 degrees C for one day, and finally recovered at 10 degrees C. The measurement of physiological parameters, including superoxide dismutase, peroxidase, malondialdehyde, thiobarbituric acid, and soluble sugar, showed that the physiological change of acclimated plants is smoother than non-acclimated plants. The RNA-Seq ana-lyses pointed out that cold acclimation already fixed the different gene expression patterns of D. odorifera T. Chen trees. The hormone-related, secondary metabolic, and signal transduction related biological genes tend to show different expressions between the acclimated and non-acclimated D. odorifera T. Chen trees. Moreover, the change of gene expression for some biological processes, such as alpha-Linolenic acid metabolism and its response to hydrogen peroxide, seems to occur earlier in non-acclimated than acclimated plants. The ISOSeq analyses pointed out that alternative splicing (AS) of some genes was also found, and these AS events were predicted to play important roles in regulating different expression patterns between non-acclimated and acclimated plants. Therefore, according to the performance of this study, we are able to provide some novel understanding of cold tolerance enhanced by cold acclimation in perennial trees.

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