Barley yellow dwarf virus-GAV 17K protein disrupts thiamine biosynthesis to facilitate viral infection in plants
文献类型： 外文期刊
作者： Han, Xiaoyu 1 ; Yang, Xue 1 ; Chen, Siyu 1 ; Wang, He 1 ; Liu, Xiaomin 2 ; Wang, Daowen 3 ; Yang, Jin 3 ; Chen, Linlin 1 ; Sun, Bingjian 1 ; Li, Honglian 1 ; Shi, Yan 1 ;
作者机构： 1.Henan Agr Univ, Coll Plant Protect, Zhengzhou 450046, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Inst Cereal & Crops, Shijiazhuang 050031, Peoples R China
3.Henan Agr Univ, Coll Agron, Zhengzhou 450046, Peoples R China
4.Natl Key Lab Wheat & Maize Crop Sci, Zhengzhou 450046, Peoples R China
关键词： barley yellow dwarf virus; 17K; thiamine; pyrimidine synthase (THIC); transcript profiling
期刊名称：PLANT JOURNAL （ 影响因子：7.2； 五年影响因子：7.9 ）
ISSN： 0960-7412
年卷期： 2024 年
页码：
收录情况： SCI
摘要： Thiamine functions as a crucial activator modulating plant health and broad-spectrum stress tolerances. However, the role of thiamine in regulating plant virus infection is largely unknown. Here, we report that the multifunctional 17K protein encoded by barley yellow dwarf virus-GAV (BYDV-GAV) interacted with barley pyrimidine synthase (HvTHIC), a key enzyme in thiamine biosynthesis. HvTHIC was found to be localized in chloroplast via an N-terminal 74-amino acid domain. However, the 17K-HvTHIC interaction restricted HvTHIC targeting to chloroplasts and triggered autophagy-mediated HvTHIC degradation. Upon BYDV-GAV infection, the expression of the HvTHIC gene was significantly induced, and this was accompanied by accumulation of thiamine and salicylic acid. Silencing of HvTHIC expression promoted BYDV-GAV accumulation. Transcriptomic analysis of HvTHIC silenced and non-silenced barley plants showed that the differentially expressed genes were mainly involved in plant-pathogen interaction, plant hormone signal induction, phenylpropanoid biosynthesis, starch and sucrose metabolism, photosynthesis-antenna protein, and MAPK signaling pathway. Thiamine treatment enhanced barley resistance to BYDV-GAV. Taken together, our findings reveal a molecular mechanism underlying how BYDV impedes thiamine biosynthesis to uphold viral infection in plants.
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