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Target gene overexpression and enhanced metabolism confer resistance to nicosulfuron in Eriochloa villosa (Thunb.)

文献类型： 外文期刊

作者： Han, Yujun 1 ; Gao, Hong 1 ; Sun, Ying 1 ; Wang, Yuechao 2 ; Yan, Chunxiu 1 ; Ma, Hong 1 ; Liu, Xiaomin 3 ; Huang, Zhaofeng 4 ;

作者机构： 1.Northeast Agr Univ, Coll Plant Protect, Harbin 150030, Peoples R China

2.Heilongjiang Acad Agr Sci, Crop Resources Inst, Harbin 150086, Peoples R China

3.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Key Lab Crop Cultivat Physiol & Green Prod Hebei P, Shijiazhuang 050035, Peoples R China

4.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

关键词： Eriochloa villosa; Acetolactate synthase (ALS); Metabolic resistance; Cross resistance; Target-site resistance

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 0048-3575

年卷期： 2024 年 202 卷

页码：

收录情况： SCI

摘要： Eriochloa villosa (Thunb.) Kunth is a troublesome weed widely distributed in maize (Zea mays L.) fields in Northeast China. Many populations of E. villosa have evolved resistance to nicosulfuron herbicides, which inhibit acetolactate synthase (ALS). The objectives of this research were to confirm that E. villosa is resistant to nicosulfuron and to investigate the basis of nicosulfuron resistance. Whole-plant dose-response studies revealed that the R population had not developed a high level of cross-resistance and exhibited greater resistant (25.62-fold) to nicosulfuron than that of the S population and had not yet developed a high level of cross-resistance. An in vitro ALS activity assay demonstrated that the I50 of nicosulfuron was 6.87-fold greater in the R population than the S population. However, based on ALS gene sequencing, the target ALS gene in the R population did not contain mutations. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed that ALS gene expression between the R and S populations was significantly different after nicosulfuron application, but no differences were observed in the gene copy number. After the cytochrome P450 inhibitor malathion or the GST inhibitor NBD-Cl was applied, the resistant E. villosa population exhibited increased sensitivity to nicosulfuron. Based on the activities of GSTs and P450s, the activities of the R population were greater than those of the S population after nicosulfuron application. This is the first report that the resistance of E. villosa to ALS inhibitors results from increased target gene expression and increased metabolism. These findings provide a theoretical foundation for the effective control of herbicide-resistant E. villosa.

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