Hybrid RSM-GA approach to optimize extraction conditions for blueberry anthocyanins with high antioxidant activity
文献类型： 外文期刊
作者： Fang, Xiangjun 1 ; Wu, Weijie 2 ; Mu, Honglei 2 ; Chen, Hangjun 2 ; Zheng, Xiaolin 1 ; Gao, Haiyan 2 ;
作者机构： 1.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou, Peoples R China
2.Zhejiang Acad Agr Sci, Food Sci Inst, China Key Lab Postharvest Handling Fruits, Minist Agr & Rural Affairs,Key Lab Fruits & Veget, Hangzhou, Peoples R China
3.China Natl Light Ind, Key Lab Postharvest Preservat & Proc Fruits & Veg, Hangzhou, Peoples R China
关键词： Blueberry; anthocyanins; response surface methodology; genetic algorithm; antioxidant
期刊名称：JOURNAL OF BERRY RESEARCH （ 影响因子：1.7； 五年影响因子：1.8 ）
ISSN： 1878-5093
年卷期： 2023 年 13 卷 2 期
页码：
收录情况： SCI
摘要： BACKGROUND: Blueberry contains an abundance of anthocyanins, which are a bioactive component of this fruit. Anthocyanins can be extracted via various methods, and each has pros and cons. OBJECTIVE: This current study reported the optimal conditions for the ultrasonic-assisted enzymatic extraction of blueberry anthocyanins simulated using response surface methodology (RSM) coupled with a genetic algorithm (GA). METHODS: The Box-Behnken design (BBD) was used for the RSM, and the extraction conditions were as follows: temperature, 42 degrees C; ultrasonic power, 310 W; enzyme volume, 0.25%; and extraction time, 42 min. RESULTS: The maximum predicted extraction yield was 6.67 mg/g. The antioxidant activity of anthocyanins extracted via RSM and GA was based on the hydroxyl free radical activity and supersonic anion free radical activity of 230.50 +/- 12.76 mu g/ml and 4.41 +/- 0.36 mu g/ml, respectively. Anthocyanins exracted by the proposed method has stronger free radical removal capacity than that of Vc. CONCLUSIONS: This study shows that the combination of RSM with GA represents an optimized method for extracting blueberry anthocyanins for use in the food industry. This method can maintain high antioxidant potential and can be used as an alternative strategy for high-value products.
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