Rapid, simple and direct detection of Meloidogyne hapla from infected root galls using loop-mediated isothermal amplification combined with FTA technology
文献类型： 外文期刊
作者： Peng, Huan 1 ; Long, Haibo 2 ; Huang, Wenkun 1 ; Liu, Jing 1 ; Cui, Jiangkuan 1 ; Kong, Lingan 1 ; Hu, Xianqi 3 ; Gu, Jia 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
2.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China
3.Yunnan Agr Univ, Natl Engn Res Ctr Agribiodivers Appl Technol, Kunming 650201, Yunnan, Peoples R China
4.Acad Inspect & Quarantine, Ningbo 315012, Zhejiang, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2017 年 7 卷
页码：
收录情况： SCI
摘要： The northern root-knot nematode (Meloidogyne hapla) is a damaging nematode that has caused serious economic losses worldwide. In the present study, a sensitive, simple and rapid method was developed for detection of M. hapla in infested plant roots by combining a Flinders Technology Associates (FTA) card with loop-mediated isothermal amplification (LAMP). The specific primers of LAMP were designed based on the distinction of internal transcribed spacer (ITS) sequences between M. hapla and other Meloidogyne spp. The LAMP assay can detect nematode genomic DNA at concentrations low to 1/200 000, which is 100 times more sensitive than conventional PCR. The LAMP was able to highly specifically distinguish M. hapla from other closely related nematode species. Furthermore, the advantages of the FTA-LAMP assay to detect M. hapla were demonstrated by assaying infected root galls that were artificially inoculated. In addition, M. hapla was successfully detected from six of forty-two field samples using FTA-LAMP technology. This study was the first to provide a simple diagnostic assay for M. hapla using the LAMP assay combined with FTA technology. In conclusion, the new FTA-LAMP assay has the potential for diagnosing infestation in the field and managing the pathogen M. hapla.
- 相关文献
作者其他论文 更多>>
-
Detection and molecular characterization of the seville root-knot nematode Meloidogyne hispanica parasiting pepper in China
作者：Wang, Li;Wang, Li;Chen, Yuan;Wang, Shuaili;Feng, Tuizi;Sun, Yanfang;Long, Haibo
关键词：Root-knot nematode; Meloidogyne hispanica; Pepper; Characterization
-
Identification and molecular characterization of two novel viruses from Allamanda cathartica in China
作者：Che, Haiyan;Ma, Yuxin;Long, Haibo;Che, Haiyan;Long, Haibo;Wu, Yangxin
关键词：
-
Morphological and Molecular Characterization of a New Root-Knot Nematode, Meloidogyne limonae n. sp. (Nematoda: Meloidogynidae), Parasitizing Lemon in China
作者：Sun, Yangfang;Long, Haibo;Jiang, Ru;Peng, Deliang;Peng, Huan;Zhang, Yan
关键词：Citrus limon; histopathology; nematode taxonomy; root-knot nematode
-
As a Transitional Host, Weed Solanum nigrum L. Increases the Population Base of Root-Knot Nematode Meloidogyne enterolobii for the Next Season
作者：Pei, Yueling;Sun, Yanfang;Chen, Yuan;Feng, Tuizi;Che, Haiyan;Long, Haibo;Long, Haibo
关键词：Meloidogyne enterolobii; transitional host; Solanum nigrum L.; parasitic characteristics; population base
-
Brown planthopper infestation on rice reduces plant susceptibility to Meloidogyne graminicola by reducing root sugar allocation
作者：Xiao, Liying;Xiao, Xueqiong;Guo, Xiaoli;Yang, Lijie;Wang, Gaofeng;Xiao, Liying;Xiao, Xueqiong;Xu, Lihe;Guo, Xiaoli;Yang, Lijie;Ma, Kangsheng;Wang, Gaofeng;Xiao, Yannong;Gheysen, Godelieve;Yang, Mingwei;Liu, Wen;He, Yueping;Peng, Deliang;Peng, Huan;Long, Haibo
关键词：Nilaparvata lugens; oviposition; phloem-feeding; rice root-knot nematode; sugar allocation; tripartite interaction
-
Effects of low-molecular-weight organic acids on the transformation and phosphate retention of iron (hydr)oxides
作者：Liang, Yu;Liu, Jing;Jin, Jiezi;Wei, Zhiyuan;Liu, Jing;Jin, Jiezi
关键词：Phosphate availability; Root-exudated LMWOAs; Rhizobox simulation; Iron (hydr)oxide
-
Occurrence of Root-Knot Nematode (Meloidogyne spp.) on Peppers in Hainan, China, and M. enterolobii and M. incognita Resistance of Common Cultivars
作者：Long, Haibo;Sun, Yanfang;Chen, Yuan;Pei, Yueling;Feng, Tuizi;Che, Haiyan;Long, Haibo
关键词：Meloidogyne enterolobii; occurrence; pepper; resistance; root-knot nematodes
