bta-miR-23a involves in adipogenesis of progenitor cells derived from fetal bovine skeletal muscle
文献类型： 外文期刊
作者： Guan, Long 1 ; Hu, Xin 1 ; Liu, Li 2 ; Xing, Yishen 1 ; Zhou, Zhengkui 1 ; Liang, Xingwei 3 ; Yang, Qiyuan 4 ; Jin, Sheng 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
2.Heilongjiang Acad Agr Sci, Inst Anim Husb, Harbin 150086, Peoples R China
3.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangxi High Educ Lab Anim Reprod & Biotechnol, Guangxi 530004, Peoples R China
4.Washington State Univ, Dept Anim Sci, Pullman, WA 99164 USA
5.Anim Husb Stn Wulagai, Wulagai 026321, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2017 年 7 卷
页码：
收录情况： SCI
摘要： Intramuscular fat deposition or marbling is essential for high quality beef. The molecular mechanism of adipogenesis in skeletal muscle remains largely unknown. In this study, we isolated Platelet-derived growth factor receptor alpha (PDGFR alpha) positive progenitor cells from fetal bovine skeletal muscle and induced into adipocytes. Using miRNAome sequencing, we revealed that bta-miR-23a was an adipogenic miRNA mediating bovine adipogenesis in skeletal muscle. The expression of bta-miR-23a was down-regulated during differentiation of PDGFR alpha+ progenitor cells. Forced expression of bta-miR-23a mimics reduced lipid accumulation and inhibited the key adipogenic transcription factor peroxisome proliferative activated receptor gamma (PPAR gamma) and CCAAT/enhancer binding protein alpha (C/EBP alpha). Whereas down-regulation of bta-miR-23a by its inhibitors increased lipid accumulation and expression of C/EBP alpha, PPAR gamma and fatty acid-binding protein 4 (FABP4). Target prediction analysis revealed that ZNF423 was a potential target of bta-miR-23a. Dual-luciferase reporter assay revealed that bta-miR-23a directly targeted the 3'-UTR of ZNF423. Together, our data showed that bta-miR-23a orchestrates early intramuscular adipogeneic commitment as an anti-adipogenic regulator which acts by targeting ZNF423.
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