文献类型： 外文期刊

作者： Xie, L. B. ¹ ; Wang, X. ¹ ; Peng, M. ² ; Zhou, Y. ¹ ; Chen, L. X. ¹ ; Liu, L. X. ³ ; Gao, Y. L. ¹ ; Guo, Y. H. ¹ ;

作者机构： 1.Heilongjiang Acad Agr Sci, Hort Sub Acad, Harbin 150069, Heilongjiang, Peoples R China

2.Northeast Forestry Univ, Coll Life Sci, Harbin 150040S, Heilongjiang, Peoples R China

3.Chinese Acad Agr Sci, Natl Ctr Space Mutagenesis Crop Improvement, Inst Crop Sci, Beijing 100081, Peoples R China

关键词： Capsicum annuum L.;Proteomics;Space flight

期刊名称：PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：1.039； 五年影响因子：0.77 ）

ISSN： 1851-5657

年卷期： 2017 年 86 卷

页码：

收录情况： SCI

摘要： Hot pepper (Capsicum annuum L.) is an important crop all over the world. To explore and identify differentially expressed proteins of hot pepper after space flight, three space-induced mutants (Y1, Y2 and Y3), which obtained new traits after space flight compared with their control lines (W1 and W2), were analyzed using comparative proteome analysis. In this study, leaf morphological characteristics of five kinds of hot pepper variations were evaluated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results showed that obvious changes of cellular structure were seen in space induced mutants. Thirty nine out of the 255 differentially expressed proteins were successfully sequenced. Among these 39 proteins, 31 were homologous with known proteins, and the others were set as a hypothetical protein. All identified proteins were further classified into six groups including protein metabolism, energy metabolism and photosynthesis. In conclusion, these findings will benefit for the research of mutagenic mechanisms at the level of proteomics, and provide an effective way for new hot pepper breeding.