Analysis of the Synergistic Effect of Endophytic Bacteria and Metabolites on Yield in Soybean Roots Under Biochar with Nitrogen Fertilization
文献类型： 外文期刊
作者： Liu, Hui 1 ; Dou, Wanyu 2 ; Zhang, Wenlong 2 ; Li, Yutao 3 ; Shi, Guoxin 4 ; Fu, Lifang 1 ; Wang, Lihong 5 ;
作者机构： 1.Northeast Agr Univ, Coll Arts & Sci, Harbin 150030, Peoples R China
2.Northeast Agr Univ, Coll Engn, Harbin 150030, Peoples R China
3.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150010, Peoples R China
4.Northeast Agr Univ, Coll Agr, Harbin 150030, Peoples R China
5.Northeast Agr Univ, Coll Water Resources & Civil Engn, Harbin 150030, Peoples R China
关键词：
biochar; nitrogen fertilizer; endophytic bacteria; metabolomics;
期刊名称：AGRONOMY-BASEL （ 影响因子：3.4； 五年影响因子：3.8 ）
ISSN：
年卷期： 2025 年 15 卷 1 期
页码：
收录情况： SCI
摘要： The appropriate application of nitrogen fertilizers and biochar to the soil is beneficial for soybean growth, and a combination of biochar and nitrogen can improve low-nitrogen fertilizer utilization efficiency. However, the effect of the combination of biochar and nitrogen fertilizer on soybean roots is still unclear. Therefore, in this study, we investigate the changes in endophytic bacterial communities and metabolites in soybean roots under different combinations of biochar and nitrogen fertilizer treatments by setting different fertilization levels and using high-throughput sequencing and non-targeted metabolomics techniques. The results showed that applying 20 t/ha of biochar and 180 kg/ha of nitrogen fertilizer (C2N1) resulted in the highest soybean yield. By studying the endophytic bacterial community in soybean roots, it was observed that the dominant phyla were Proteobacteria, Bacteroidota, and Actinobacteriota, and the dominant genera were Bradyrhizobium, Streptomyces, and Rhizobacter. Moreover, a metabolic pathway analysis revealed that glycosylphosphatidylinositol (GPI)-anchor biosynthesis was the most significant metabolic pathway in the C0N1 vs. C0N0 comparison group, and glycerophospholipid metabolism was the most significant metabolic pathway in the C2N1 vs. C1N1 comparison group; they were both associated with regulatory mechanisms such as plant growth promotion and nutrient cycling. Structural equation modeling indicates that both endophytic bacteria and metabolites have a significant positive impact on yield. Bradyrhizobium and Rhizobium are observable variables with significant positive effects on endophytic bacteria, while Phe-Thr and 7-allyloxycoumarin are observable variables with significant positive effects on metabolites. Endophytic bacteria not only have a direct impact on crop yield, but also indirectly affect crop yield through their effects on metabolites. This study has important scientific value and guiding significance for improving soybean yields.
- 相关文献
作者其他论文 更多>>
-
Dynamic Impacts of Stock Enhancement on Kaluga Sturgeon (Huso dauricus): Novel Conservation Strategy Insights from the Gut Microbe Composition and Gene Expression Mode
作者：Li, Yutao;Wang, Ruoyu;Cao, Dingchen;Zhang, Ying;Zhai, Cunhua;Sun, Zhipeng;Ma, Bo
关键词：stock enhancement; sturgeon; environmental adaptation; gut microbiota; conservation biology
-
Carbon footprint of shrimp (Litopenaeus vannamei) cultured in recirculating aquaculture systems (RAS) in China
作者：Rong, Fan;Liu, Hui;Zhu, Jianxin;Qin, Gaoju;Rong, Fan
关键词：Carbon footprint; Life cycle assessment; Recirculating aquaculture system; Litopenaeus vannamei
-
Chronic Heat Stress Caused Lipid Metabolism Disorder and Tissue Injury in the Liver of Huso dauricus via Oxidative-Stress-Mediated Ferroptosis
作者：Zhang, Yining;Li, Yutao;Wang, Ruoyu;Wang, Sihan;Sun, Bo;Cao, Dingchen;Sun, Zhipeng;Lv, Weihua;Zhang, Ying;Zhang, Yining;Ma, Bo;Zhang, Ying;Ma, Bo
关键词：Huso dauricus; oxidative stress; heat stress; liver; transcriptome; kaluga sturgeon
-
Coapplication of biochar and nitrogen fertilizer affects soybean yield by regulating the microbiome and metabolite profile of rhizosphere soil
作者：Liu, Hui;Dou, Wanyu;Zhang, Wenlong;Shi, Guoxin;Li, Yutao;Wang, Lihong
关键词：Biochar; Nitrogen fertilizer; High-throughput sequencing; Metabolomics
-
Combined Impacts of Acute Heat Stress on the Histology, Antioxidant Activity, Immunity, and Intestinal Microbiota of Wild Female Burbot (Lota Lota) in Winter: New Insights into Heat Sensitivity in Extremely Hardy Fish
作者：Zhai, Cunhua;Li, Yutao;Wang, Ruoyu;Han, Haoxiang;Zhang, Ying;Ma, Bo;Zhai, Cunhua;Han, Haoxiang;Ma, Bo;Li, Yutao;Wang, Ruoyu;Zhang, Ying
关键词：
lota lota ; heat stress; antioxidant; immune; apoptosis; microbiomics -
Differential Cell Death Pathways Induced by Oxidative Stress in Multi-Organs of Amur Grayling (Thymallus grubii) Under Gradient Ammonia Stress
作者：Zhai, Cunhua;Li, Yutao;Wang, Ruoyu;Zhang, Ying;Ma, Bo;Zhai, Cunhua;Li, Yutao;Wang, Ruoyu;Zhang, Ying;Ma, Bo;Ma, Bo
关键词：ammonia;
Thymallus grubii ; oxidative stress; multi-omics; intestinal microbe; cell death -
Excessive heavy metal enrichment disturbs liver functions through the gut microbe in the great Himalayan leaf-nosed bat (Hipposideros armiger)
作者：Li, Yutao;Peng, Jie;Cheng, Zheng;Zhang, Kangkang;Gu, Hao;Feng, Jiang;Liu, Ying;Li, Yutao;Feng, Jiang;Zhang, Kangkang;Feng, Jiang;Liu, Ying;Liu, Ying
关键词：Heavy metals; Bat; Hepatotoxicity; Metabolism disorder; Conservation biology
