您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effects of Pore Size and Crosslinking Methods on the Immobilization of Myoglobin in SBA-15

文献类型： 外文期刊

作者： Miao, Hengmin 1 ; Li, Maosheng 2 ; Sun, Xiaochun 4 ; Xia, Jikun 2 ; Li, Yanqing 2 ; Li, Jiao 1 ; Wang, Fang 2 ; Xu, Jiakun 2 ;

作者机构： 1.Shandong Univ Technol, Sch Mat Sci & Engn, Zibo, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fisheries, Lab Marine Drugs & Byprod Pilot,Natl Lab Marine S, Qingdao, Peoples R China

3.Qilu Univ Technol, Shandong Acad Sci, Sch Food Sci & Engn, Jinan, Peoples R China

4.Marine Sci Res Inst Shandong Prov, Qingdao, Peoples R China

5.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao, Peoples R China

6.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai, Peoples R China

关键词： myoglobin; SBA-15; immobilization; catalytic activity; reusability

期刊名称：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY （ 影响因子：6.064； 五年影响因子：6.303 ）

ISSN： 2296-4185

年卷期： 2022 年 9 卷

页码：

收录情况： SCI

摘要： A series of stable mesoporous silica sieves (SBA-15) with different pore sizes (9.8, 7.2, and 5.5 nm) were synthesized using a hydrothermal method. The resulting mesoporous material was then utilized for protein immobilization using myoglobin (Mb) as the target protein. The effects of pore size and adsorption methods on the immobilization efficiency of Mb in a mesoporous material were studied. The SBA-15 with a pore size of 7.2 nm showed the best loading capacity, reaching 413.8 mg/g. The SBA-15 with a pore size of 9.8 nm showed the highest retained catalytic ability (92.36%). The immobilized enzyme was more stable than the free enzyme. After seven consecutive assay cycles, Mb adsorbed by SBA-15 (Mb/SBA-15) and Mb adsorbed by SBA-15 and crosslinked with glutaraldehyde (Mb/G/SBA-15) retained 36.41% and 62.37% of their initial activity, respectively.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台