Effects of Pore Size and Crosslinking Methods on the Immobilization of Myoglobin in SBA-15
文献类型： 外文期刊
作者： Miao, Hengmin 1 ; Li, Maosheng 2 ; Sun, Xiaochun 4 ; Xia, Jikun 2 ; Li, Yanqing 2 ; Li, Jiao 1 ; Wang, Fang 2 ; Xu, Jiakun 2 ;
作者机构： 1.Shandong Univ Technol, Sch Mat Sci & Engn, Zibo, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fisheries, Lab Marine Drugs & Byprod Pilot,Natl Lab Marine S, Qingdao, Peoples R China
3.Qilu Univ Technol, Shandong Acad Sci, Sch Food Sci & Engn, Jinan, Peoples R China
4.Marine Sci Res Inst Shandong Prov, Qingdao, Peoples R China
5.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao, Peoples R China
6.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai, Peoples R China
关键词： myoglobin; SBA-15; immobilization; catalytic activity; reusability
期刊名称：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY （ 影响因子：6.064； 五年影响因子：6.303 ）
ISSN： 2296-4185
年卷期： 2022 年 9 卷
页码：
收录情况： SCI
摘要： A series of stable mesoporous silica sieves (SBA-15) with different pore sizes (9.8, 7.2, and 5.5 nm) were synthesized using a hydrothermal method. The resulting mesoporous material was then utilized for protein immobilization using myoglobin (Mb) as the target protein. The effects of pore size and adsorption methods on the immobilization efficiency of Mb in a mesoporous material were studied. The SBA-15 with a pore size of 7.2 nm showed the best loading capacity, reaching 413.8 mg/g. The SBA-15 with a pore size of 9.8 nm showed the highest retained catalytic ability (92.36%). The immobilized enzyme was more stable than the free enzyme. After seven consecutive assay cycles, Mb adsorbed by SBA-15 (Mb/SBA-15) and Mb adsorbed by SBA-15 and crosslinked with glutaraldehyde (Mb/G/SBA-15) retained 36.41% and 62.37% of their initial activity, respectively.
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