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Dietary leucine modulates growth performance, Nrf2 antioxidant signaling pathway and immune response of juvenile blunt snout bream (Megalobrama amblycephala)

文献类型: 外文期刊

作者: Liang, Hualiang 1 ; Mokrani, Ahmed 1 ; Ji, Ke 1 ; Ge, Xianping 1 ; Ren, Mingchun 1 ; Xie, Jun 1 ; Liu, Bo 1 ; Xi, Bingwen 2 ;

作者机构: 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China

2.CAFS, Key Lab Genet Breeding Aquat Anim & Aquaculture B, FFRC, Wuxi 214081, Peoples R China

3.CAFS, Key Lab Genet Breeding Aquat Anim & Aquaculture B, FFRC, Wuxi 214081,

关键词: Blunt snout bream (Megalobrama amblycephala);leucine;Growth performance;Nrf2 antioxidant signaling pathway;Immune response

期刊名称:FISH & SHELLFISH IMMUNOLOGY ( 影响因子:4.581; 五年影响因子:4.851 )

ISSN: 1050-4648

年卷期: 2018 年 73 卷

页码:

收录情况: SCI

摘要: The present study assessed the effects of dietary leucine on growth performance, antioxidant status and immunity in juvenile blunt snout bream. Fish were fed six practical diets of graded leucine levels ranging from 0.90% to 2.94% of dry basis for 8 weeks. Trail results showed that compared to control group (0.90%), 1.72% dietary leucine level significantly improved final weight (FW), weight gain rate (WG) and specific growth rate (SGR), and significantly lowered feed conversion ratio (FCR). Based on WG and SGR, the optimal dietary leucine level was obtained at 1.40% and 1.56%, respectively. Whole body crude lipid and protein contents were improved with increasing dietary leucine up to 2.14% and thereafter showed a downward trend, while whole body moisture content showed a converse trend. No significant change was found in whole body ash content. 1.72% dietary leucine level significantly improved the antioxidant capacity of fish by regulating the plasma superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, total antioxidant capacity (T-AOC) activity, catalase (CAT) activity, aspartate aminotransferase (AST) activities and malondialdehyde (MDA) content, furthermore, 1.72% dietary leucine level also significantly improved the antioxidant genes expressions of associated with Nrf2 signaling pathway by regulating heme oxygenase-1 (HO-1), GPx, copperezinc superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (Mn-SOD), 2.14% dietary leucine levels also significantly improved glutathione transferase (GST) mRNA level. Dietary leucine levels significantly affected plasma immunity parameters such as the contents of plasma complement component 3 (C3), immunoglobulin M (IgM) and lowered the hepatopancreas genes expressions of pro-inflammatory factor by regulating interleukin 1 beta (IL-1 beta), interleukin 8 (IL-8) and tumour necrosis factor-alpha (TNF-alpha) mRNA levels. The present study indicated that optimal dietary leucine level plays an important role in improving growth, enhancing antioxidant and immune status to maintain the health in juvenile blunt snout bream.

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